| RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila （Isoetaceae） |
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| 作者姓名： | CHENJin-ming WANGJing-yuan LIUXing GituruWahitiRobert WANGQing-feng |
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| 作者单位： | [1]LaboratoryofPlantSystematicsandEvolutionaryBiology／CollegeofLifeSciences,WuhanUniversity,Wuhan430072,Hubei,China [2]BotanyDepartment,KenyattaUniversity,P.O.Box43844,Nairobi,Kenya |
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| 摘 要： | The genetie variation in the critically endangered species Isoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104 DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69% to 25.96% was found. An Analysis of Molecular Variance (AMOVA) indicated that the most of variance (78.30%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations were only 3.89% and 17.82%, respectively.
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| 关 键 词： | RAPD分析 遗传变异 水韭属植物 生物特征 基因 |
| 收稿时间： | 19 February 2004 |
RAPD analysis for genetic variation within the endangered quillwortIsoetes hypsophila (isoetaceae) |
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| CHENJin-ming WANGJing-yuan LIUXing GituruWahitiRobert WANGQing-feng.RAPD analysis for genetic variation within the endangered quillwortIsoetes hypsophila (isoetaceae)[J].Wuhan University Journal of Natural Sciences,2005,10(2):455-459. |
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| Authors: | Chen Jin-ming Wang Jing-yuan Liu Xing Gituru Wahiti Robert Wang Wing-feng |
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| Institution: | (1) Laboratory of Plant Systematics and Evolutionary Biology/College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, China;(2) Botany Department, Kenyatta University, P. O. Box, 43844 Nairobi, Kenya |
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| Abstract: | The genetic variation in the critically endangered speciesIsoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers,
and a total of 104 DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations
with PPB values ranging from 7.69% to 25.96% was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most
of variance (78.30%) occurred between Yunnan and Si chuan. The variances among populations within regions and within populations
were only 3.89% and 17.82%, respectively.
Foundation item: Supported by the National Natural Science Foun dation of China (30370098) and the State Key Basic Research and Development
Plan (G2000046805)
Biography: CHEN Jin-ming (1975), male, Ph. D candidate, research direction: conservation biology. |
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| Keywords: | conservation endangered genetic diversity Isoetes hypsophila RAPI |
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