| Ebselen 抑制 ONOO~- 对神经元[Ca~(2+)]_i 的影响 |
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| 引用本文： | 易永,蔡东,瞿安连,黄开勋.Ebselen 抑制 ONOO~- 对神经元[Ca~(2+)]_i 的影响[J].华中科技大学学报(自然科学版),1998(5). |
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| 作者姓名： | 易永 蔡东 瞿安连 黄开勋 |
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| 作者单位： | 华中理工大学生物物理与生物化学研究所 |
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| 摘 要： | Ｆｕｒａ－２显微荧光测钙技术研究发现，过氧亚硝基阴离子（ＯＮＯＯ－）作用于ＭＮ９Ｄ细胞，数ｓ内即可导致其胞内游离钙离子浓度（［Ｃａ２＋］ｉ）的急剧升高．胞外液换为无钙液或向胞外液中加入硝苯吡啶（Ｎｉｆｅｄｉｐ－ｉｎｅ）、二硫苏糖醇（ＤＴＴ）均可抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响，提示Ｌ－型钙通道的激活是ＯＮＯＯ－引起［Ｃａ２＋］ｉ升高的主要原因，ＯＮＯＯ－的这种作用可能与其氧化特性有关．Ｅｂｓｅｌｅｎ（２－苯基－１，２－苯并异硒唑－３（２Ｈ）酮）明显抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响，并且存在一定的剂量效应关系．
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| 关 键 词： | 过氧亚硝基阴离子 胞内游离钙离子浓度 MN9D细胞 Ebselen |
Ebselen Inhibiting the Effect of ONOO - on [Ca 2+ ] i in Neuronal Cell |
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| Yi Yong,Ph. D..Ebselen Inhibiting the Effect of ONOO - on [Ca 2+ ] i in Neuronal Cell[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,1998(5). |
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| Authors: | Yi Yong Ph D |
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| Institution: | Yi Yong Ph. D., Institute of Biophysics & Biochemistry,HUST,Wuhan 430074,China. Cai Dong Qu Anlian Huang Kaixun |
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| Abstract: | The mechanism of peroxynitrite (ONOO -) induced Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by Fura 2 microfluorometric technique. Results showed that ONOO - caused a rapid increase of Ca 2+ ] i when it was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (Nifedipine) greatly inhibited the Ca 2+ ] i increase induced by ONOO -, which suggests that the opening of L Ca 2+ channel makes a great contribution to the Ca 2+ ] i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO - induced Ca 2+ ] i increase suggests that ONOO - activating L Ca 2+ channel is partly related to its oxidative speciality. Ebselen shows an inhibiting effect on ONOO - induced Ca 2+ ] i increase. |
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| Keywords: | peroxynitrite (ONOO -) intracellular calcium concentration MN9D cell ebselen |
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