| 真核原核两界细胞融合子SEM形态 |
| |
| 引用本文： | 陈文权,严俊,陶菁,陈逊,裴大平,顾继东,王莹莹,裘婉飞,范延臻,程树培.真核原核两界细胞融合子SEM形态[J].南京大学学报(自然科学版),2002,38(4):550-555. |
| |
| 作者姓名： | 陈文权 严俊 陶菁 陈逊 裴大平 顾继东 王莹莹 裘婉飞 范延臻 程树培 |
| |
| 作者单位： | [1]南京大学污染控制与资源化研究国家重点实验室、环境学院，南京210093 [2]香港大学生态学与分类学系，香港 |
| |
| 基金项目： | 国家 8 6 3课题 (2 0 0 1AA2 14 191)，江苏省自然科学基金 (BK990 33)，香港中国环保工程公司资助研究 |
| |
| 摘 要： | 报道应用扫描电子显微镜（SEM）、测定跨界融合Fhhh细胞形态的结果。Fhhh由2株真菌真核细胞与1株细菌原核细胞的原生质体融合而成。2株真菌是黄孢原毛平革菌和酿酒酵母，1株细菌是从对苯二甲酸废水活性污泥中筛选获得的土细菌。3个亲株菌体细胞SEM的形态差异极为显，既不同于黄孢原毛平革菌与土细胞的首次跨界融合获得的Fhh，也不同于Fhh与酿酒酵母的2次跨界融合获得的Fhhh。经过350多代的繁殖传代。Fhhh仍然同时含有来自3亲株的基因DNA，具有分子遗传稳定性。研究结果表明，跨界原生质体融合是一种快速有效的构建基因工程菌的生物技术。
|
| 关 键 词： | 细胞融合子 黄孢原毛平革菌 酿酒酵母 土著细菌 原生质体融合 SEM 细胞形态 遗传稳定性 真核细胞 原核细胞 |
Morphological Identification with SEM for the Fusant Fhhh Constructed from Eukaryote and Prokaryote Strains |
| |
| Chen Wenquan ,Yan Jun ,Tao Jing ,Chen Xun ,Pei Daping l,Gu Jidong ,Wang Yingying ,Qiu Wanfei ,Fan Yanzhen ,Cheng Shupei.Morphological Identification with SEM for the Fusant Fhhh Constructed from Eukaryote and Prokaryote Strains[J].Journal of Nanjing University: Nat Sci Ed,2002,38(4):550-555. |
| |
| Authors: | Chen Wenquan Yan Jun Tao Jing Chen Xun Pei Daping l Gu Jidong Wang Yingying Qiu Wanfei Fan Yanzhen Cheng Shupei |
| |
| Institution: | Chen Wenquan 1,Yan Jun 1,Tao Jing 1,Chen Xun 1,Pei Daping l,Gu Jidong 2,Wang Yingying 2,Qiu Wanfei 2,Fan Yanzhen 2,Cheng Shupei 1 |
| |
| Abstract: | The morphological identification with SEM for the inter_kingdom fusant Fhhh was conducted in this research. Fhhh was the result of the protoplast fusion of three parental strains: the fungous Phanerochaete chrysosporium, the fungous Saccharomyces cerevisiae, and the native bacterium strain YZ1. YZ1 was isolated from the activated sludge of a purified terephthalic acid (PTA) wastewater treatment plant. The morphology of Fhhh with SEM was quite different from those of its 3 parental strains, and the 3 parental strains were different from each other obviously. After over 350 generations, Fhhh still contained the three gene DNA fragments existing in the three parental strains. Fhhh had genetic stability, resistance to both bacteria and fungi, and potential for the treatment of PTA wastewater. |
| |
| Keywords: | Phanerochaete chrysosporium Saccharomyces cerevisiae native bacterium protoplast fusion SEM morphology genetic stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
