犬门腔静脉吻合术的操作及在实验医学的用途

31条犬施行门腔静脉吻合术,术后24小时内死亡6条,其他25条均获长期存活。经验与体会是:吻合口位置宜低,作吻合处的下腔静脉和门静脉分支仅游离其两旁的浆膜;用心耳钳阻断门腔静脉血流,且钳刃越薄越好;吻合口前后壁均用一般连续缝合;吻合口必须大于1.5公分。本手术在实验医学的用途是:作为外科性急性肝昏迷动物模型的第一步造型手术;研究慢性肝脑病时中枢神经组织化学和病理形态学改变;研究肝移植供肝灌注液的最佳温度和成分;研究和选择用于肝保存的细胞膜稳定剂;研究血浆各种氨基酸在肝脑病时的作用。

MANIPULATION OF THE PORTOCAVAL ANASTOMOSIS IN DOG AND ITS USAGE IN THE EXPERIMENTAL MEDICINE

The experience in the manipulation of the portocaval anastomosis in 31 dogs were reported. 6 dogs died within 24 hours after operation, 25 gained long survival. Our experience were: it is better choice to locate anastomostic stoma in the lower position of the inferior vena cava. The serosa of the inferior vena and the portal vein on both sides around the anastomostic stoma was carefully separated. The blood stream was better blocked with the Harken's clamp, of which the more thinner of the edges is the better. The anastomotic stoma must be larger than 1.5 cm. The superiority of this operation were: it serves to make a model of the surgical acute hepatic coma in the animal as a first step; to study the changes of the histochemistry and pathomorphology of the central nervous system during the chronic hepaticoencephalopathy; to investigate the optimal temperature and constituent of the perfusate for the donor liver preservation; to research and select the cell menbrane stabilizer for the liver preservation; to analyse the effects of each role of the various amino-acids in the serum of the hepatic enceph alopathy.