| 藻胆蛋白表达条件优化的研究 |
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| 引用本文： | 王娟,王建勇,苏平,涂俊铭. 藻胆蛋白表达条件优化的研究[J]. 湖北师范学院学报(自然科学版), 2012, 0(4): 50-55 |
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| 作者姓名： | 王娟 王建勇 苏平 涂俊铭 |
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| 作者单位： | 湖北师范学院生命科学学院 |
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| 基金项目： | 湖北省自然基金(2009CDB354);湖北师范学院人才引进项目(2008F16) |
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| 摘 要： | 为了提高藻胆蛋白的表达含量,对本实验室构建的含pACYCDuet-ho1-pcyA、pETDuet-pecE-pecF、pCO-LADuet-pecA的E.coliBL21菌种的表达条件进行优化。通过单因素实验和正交实验L9（33）优化了蛋白的表达条件为：诱导表达温度为28℃,诱导表达时间为14h,诱导剂量为1.0mmol/L,藻胆蛋白最高表达含量为11.271mg/100mL.
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| 关 键 词： | 藻胆蛋白 表达 优化 |
Research on the optimization expression conditions of recombinant phycobiliprotein |
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| WANG Juan,WANG Jian-yong,SU Ping,TU Jun-ming. Research on the optimization expression conditions of recombinant phycobiliprotein[J]. Journal of Hubei Normal University(Natural Science), 2012, 0(4): 50-55 |
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| Authors: | WANG Juan WANG Jian-yong SU Ping TU Jun-ming |
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| Affiliation: | (College of Life Science,Hubei Normal University,Huangshi 435002,China) |
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| Abstract: | Objective The study is to optimize the culture conditions of recombinant phycobiliprotein expressed in E.coli BL 21.Through single factor and orthogonal design L9（33）experiments to make sure of the optimum conditions for culture condition.The results show that induce temperature 28℃,induce time 14h and inducer dose 1.0 mmol/L are the optimum conditions,and the yield of phycobiliproteins is 11.271 mg/100 mL. |
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| Keywords: | phycobiliprotein express optimization |
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