| 枯草芽孢杆菌TY7210菌株的ERIC-PCR快速鉴别 |
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| 引用本文： | 李海鹏,赵春贵,陈涛.枯草芽孢杆菌TY7210菌株的ERIC-PCR快速鉴别[J].山西大学学报(自然科学版),2006,29(1):89-92. |
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| 作者姓名： | 李海鹏 赵春贵 陈涛 |
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| 作者单位： | 山西大学,生物技术研究所,化学生物学与分子工程教育部重点实验室,山西,太原,030006 |
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| 摘 要： | 以ER IC核心序列为引物,以2株枯草芽孢杆菌基因组DNA为模板,采用PCR对T aq DNA聚合酶用量、PCR退火温度及模板DNA浓度进行优化,建立了快速鉴别芽孢杆菌的ER IC-PCR方法,并对5株芽孢杆菌进行了分析.结果表明枯草芽孢杆菌TY 7210菌株与其他芽孢杆菌菌株具有不同的指纹图谱,尤其不同于枯草芽孢杆菌(ATCC 1.1849),为芽孢杆菌的快速鉴别奠定了基础.
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| 关 键 词： | 芽孢杆菌 鉴别 |
| 文章编号： | 0253-2395(2006)01-0089-04 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005年10月19 |
Rapid Identification of Bacillus subtilis TY7210 by ERIC-PCR |
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| LI Hai-peng,ZHAO Chun-gui,CHEN Tao.Rapid Identification of Bacillus subtilis TY7210 by ERIC-PCR[J].Journal of Shanxi University (Natural Science Edition),2006,29(1):89-92. |
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| Authors: | LI Hai-peng ZHAO Chun-gui CHEN Tao |
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| Institution: | Institute of Biotechnology/Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanaxi University Taiyuan 030006,China |
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| Abstract: | To set up a rapid identification method for Bacillus subtilis,the amplified system of ERIC-PCR was optimized,by changing the dosage of Taq DNA polymerase,annealing temperature and the concentration of the template DNA.The genomic DNA from two strains of Bacillus subtilis were used as the template to amplify by the primer of central sequence of ERIC and the fingerprints of five strains of Bacillus spp.were analysised by using optimized procedure of ERIC-PCR.The results showed that Bacillus subtilisTY_(7210) is different from others,especially Bacillus subtilis ATCC 1.1849.It contributed to the quick identification Bacillus spp. |
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| Keywords: | ERIC-PCR |
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