| 免疫鸡群新城疫病毒检测和分离的研究 |
| |
| 引用本文： | 吴宝成,张红星,荆汝顶,徐振东,孙建宏,李康然.免疫鸡群新城疫病毒检测和分离的研究[J].广西科学,1998,5(1):76-79. |
| |
| 作者姓名： | 吴宝成 张红星 荆汝顶 徐振东 孙建宏 李康然 |
| |
| 作者单位： | 广西大学动物科技学院 |
| |
| 摘 要： | 以ＳＰＦ鸡抗新城疫病毒（ＮＤＶ）ＩｇＧ为包被抗体（５μｇ／ｍＬ），兔抗ＮＤＶＶｅｒｏ细胞培养纯化毒ＩｇＧ为第二抗体（１∶２００～６００），酶标记羊抗兔ＩｇＧ（１∶５０００）为指示抗体建立间接夹心ＥＬＩＳＡ，检测实验免疫鸡、攻毒鸡及现地免疫鸡口腔或泄殖腔外分泌物。结果表明：新城疫无毒力Ｖ４疫苗免疫后２ｄ，口腔内病毒抗原随机检出率为４／１０，第１０天为１０／１０；泄殖腔为０～１／１０，并持续１８ｄ以上。滴鼻攻毒后１ｄ，口腔和泄殖腔内病毒抗原检出率分别是８／１０和６／１０；第１３天，泄殖腔内病毒抗原检出阴性。ＮＤＶ灭活疫苗免疫６０ｄ后攻毒，第２天有７／２０口腔和８／２０泄殖腔检出阳性，并一直到攻毒后第１４天。非免疫对照鸡攻毒后第３天直到死亡时，口腔和泄殖腔均可检出病毒抗原。对各地临床上无任何症状的ＮＤＶ免疫鸡群检测发现，泄殖腔ＮＤＶ抗原阳性率０％～９．３％，分离到８株ＮＤＶ流行毒株，生物学试验证实这些毒株的毒力属中等毒力偏强或强毒。
|
| 关 键 词： | 新城疫病毒 免疫鸡群 检测 防制 |
| 收稿时间： | 1997/5/28 0:00:00 |
Detection and Isolation of Newcastle Disease Virus in Immunized Chickens |
| |
| Wu Baocheng,Zhang Hongxing,Jing Ruding,Xu Zhendong,Sun Jianhong and Li Kangran.Detection and Isolation of Newcastle Disease Virus in Immunized Chickens[J].Guangxi Sciences,1998,5(1):76-79. |
| |
| Authors: | Wu Baocheng Zhang Hongxing Jing Ruding Xu Zhendong Sun Jianhong and Li Kangran |
| |
| Institution: | College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005,College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005,College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005,College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005,College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005 and College of Animal Husbandry and Veterinary Medicine Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005 |
| |
| Abstract: | |
| |
| Keywords: | newcastle disease virus immunized chicken detection control |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《广西科学》浏览原始摘要信息 |
| 点击此处可从《广西科学》下载免费的PDF全文 |
|
