| 酵母超氧化物歧化酶的研究 |
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| 引用本文： | 程明哲,周桢林.酵母超氧化物歧化酶的研究[J].中山大学学报(自然科学版),1996,35(1):106-109. |
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| 作者姓名： | 程明哲 周桢林 |
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| 作者单位： | 中山大学生物化学系 |
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| 摘 要： | 从味精厂发酵废液培养的酵母中分离纯化超氧化物歧化酶（ＳＯＤ）纯化倍数达１００倍，比活１１１５０ｕ／ｍｇ蛋白，电泳纯，回收率达８０％；每公斤湿酵母菌体产超氧化物歧化酶酶活力达５０万单位．此酶分子量为３７２００，等电点为５．６．
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| 关 键 词： | 超氧化物歧化酶，羧甲基纤维素，连苯三酚自氧化 |
Studies of Superoxide Dismutase from Yeast |
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| Cheng Mingzhe, Zhou Zhenlin, Li Yang, Shi Yingyang.Studies of Superoxide Dismutase from Yeast[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1996,35(1):106-109. |
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| Authors: | Cheng Mingzhe Zhou Zhenlin Li Yang Shi Yingyang |
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| Abstract: | Superoxide dismutase from yeast was purification and characterized. The enzyme was purified to 100 fold by extraction, percipitation with actone and CM- Cellulose Column chromatography. Specific activity was 11150u/mg protein,electrophoretic purity, recovery was 80%. One kg of yeast cells possseses a total activity of superoxide Dismutase 500000 units. Its molecular weight was 37200, isoelectric point was 5. 6. The method is well suited for scale preperations. |
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| Keywords: | superoxide dismutase CM -Cellulose autoxidation of pyrogallol |
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