Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli |
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Authors: | Liu Deli Sun Xiaojie Qi Yipeng Zhu Ying Jin Tianquan |
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Institution: | (1) Institute of Virology, Wuhan University, 430072 Wuhan, China |
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Abstract: | The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and
3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned
into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd,
and the expressed amount reached maxium in 4 h with IPTG induction.
Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee
Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938) |
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Keywords: | nuclear capsid protein gene (vp39) PCR amplification gene cloning expression BmNPV |
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