Mouse neural stem cells culturedin vitro and expressing an exogenous gene |
| |
Authors: | Jiong Tang Hong Yu Lizhu Lin Haoquan Wu Shouyuan Zhao Changben Li |
| |
Institution: | (1) Institute of Genetics, School of Life Science, Fudan University, 200433 Shanghai, China |
| |
Abstract: | Neural stem cells are the multipotential, self-renewing cells in central nerve system, and play an essential role in the development
and differentiation of nerve system. Neural stem cells can be used to treat the nerve system diseases, especially, the transplantation
of neural stem cells to rescue the degenerated neural cells has become a very promising therapeutic way. We successfully cultured
neural stem cells isolated from the brains of embryonic micein vitro and determined their distribution in the E17 mice brains. The neural stem cells were transfected with adenoviral vector carrying
GFP (green fluorescence protein) gene and then highly expressed the exogenous gene. It paves the way for gene therapy of degenerative
nerve system diseases. |
| |
Keywords: | neural stem cells immunohistochemical assay adenoviral vector |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |