| Cloning and Characterization of the fecC Gene Necessary for Optimal Growth under Iron-Deficiency Conditions in the Cyanobacterium Anabaena sp. PCC 7120 |
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| 作者姓名： | xuwen-liang LIUYong-ding ZHANGCheng-cai LIJuan |
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| 作者单位： | [1]CollegeofLifeSciences,CentralChinaNormalUniversity,Wuhan430079,Hubei,China [2]InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,Hubei,China [3]CollegeofLifeSciencesandTechnology,HuazhongAgriculturalUniversity,Wuhan430070,Hubei,China [4]CollegeofLifeSciences,CentralChinaNormalUniversity,Wuhan430079,Hubei,China |
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| 摘 要： | The fecC gene encoding a putative iron (Ⅲ) dicitrate transporte rwas cloned from nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO3^- , NH1^- or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wildtype strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that the fecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed that fecC gene product is required for optimal growth under iron-deficient conditions in Anabaena sp. PCC 7120.
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| 关 键 词： | Anabaena sp．PCC 7120 fecC 铁缺乏 光合作用 基因表达 固氮菌 克隆 DNA隔离 |
| 收稿时间： | 16 August 2003 |
Cloning and characterization of the<Emphasis Type="Italic">fecC</Emphasis> gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium<Emphasis Type="Italic">Anabaena</Emphasis> sp. PCC 7120 |
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| xuwen-liang LIUYong-ding ZHANGCheng-cai LIJuan.Cloning and characterization of thefecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacteriumAnabaena sp. PCC 7120[J].Wuhan University Journal of Natural Sciences,2004,9(4):498-502. |
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| Authors: | Xu?Wen-Liang Email author" target="_blank">Liu?Yong-dingEmail author Zhang?Cheng-cai Li?Juan |
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| Institution: | (1) College of Life Sciences, Central China Normal University, 430079 Wuhan, Hubei, China;(2) Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, Hubei, China;(3) College of Life Sciences and Technology, Huazhong Agricultural University, 430070 Wuhan, Hubei, China |
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| Abstract: | ThefecC gene encoding a putative iron (III) dicitrate transporter was cloned from nitrogen-fixing cyanobacteriumAnabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO
3
−
, NH
4
+
or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared
between the mutant and the wildtype strain, the content of photosynthetic pigments in the mutant is lower than that of the
wild-type. The results of RT-PCR experiments show that thefecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed
thatfecC gene product is required for optimal growth under iron-deficient conditions inAnabaena sp. PCC 7120.
Foundation item: Supported by the National Natural Sciences Foundation of China (30070154), the Frontier Science Projects Program of the
Institute of Hydrobiology, the Chinese Academy of Sciences (220316), State Key Project on Cyanobacterial Bloom Control in
Lake Dianchi (K99-05-35-01)
Biography: XU Wen-liang (1974-), male, Ph. D, research direction: molecular genetics of cyanobacteria. |
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| Keywords: | Anabaena sp PCC 7120 fecC iron deficiency photosynthetic properties expression |
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