| 嗜酸氧化亚铁硫杆菌冷冻保藏保护剂的正交法优化 |
| |
| 引用本文： | 杨宇,张燕飞,何环,万民熙,师舞阳,邱冠周.嗜酸氧化亚铁硫杆菌冷冻保藏保护剂的正交法优化[J].中南大学学报(自然科学版),2006,37(5):891-895. |
| |
| 作者姓名： | 杨宇 张燕飞 何环 万民熙 师舞阳 邱冠周 |
| |
| 作者单位： | 中南大学,资源加工与生物工程学院,生物冶金教育部重点实验室,湖南,长沙,410083 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
| |
| 摘 要： | 以甘油、海藻糖、蔗糖和二甲亚砜为考察因素,采用正交实验方法,对嗜酸氧化亚铁硫杆菌冷冻保藏保护剂的优化配比进行研究.直接分析、因素指标分析和方差分析结果表明：在由甘油、海藻糖、蔗糖、二甲亚砜4种保护剂组成的混合保护剂中,对冷冻存活率影响由大至小的顺序为：甘油,海藻糖,蔗糖,二甲亚砜.最佳保护剂组合为100g/L甘油＋100g/L海藻糖＋180 g/L蔗糖＋50 g/L二甲亚砜,该组合能使冷冻存活率达到89%.
|
| 关 键 词： | 嗜酸氧化亚铁硫杆菌 冷冻保护剂 正交法 保藏 |
| 文章编号： | 1672-7207(2006)05-0891-05 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006年2月28日 |
Optimization of frozen storage cryoprotectant for Acidithiobacillus ferrooxidans by orthogonal experiment |
| |
| YANG Yu,ZHANG Yan-fei,HE Huan,WAN Min-xi,SHI Wu-yang,QIU Guan-zhou.Optimization of frozen storage cryoprotectant for Acidithiobacillus ferrooxidans by orthogonal experiment[J].Journal of Central South University:Science and Technology,2006,37(5):891-895. |
| |
| Authors: | YANG Yu ZHANG Yan-fei HE Huan WAN Min-xi SHI Wu-yang QIU Guan-zhou |
| |
| Institution: | School of Minerals Processing and Bioengineering, Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China |
| |
| Abstract: | The optimization of frozen storage cryoprotectants of Acidithiobacillus ferrooxidans was studied by orthogonal experimental method,in which glycerol,trehalose,sucrose and dimethylsufoxide(DMSO) were regarded as factors.The results of direct-viewing analysis,factor response analysis and variance analysis indicate that glycerol plays greater effect on the livability of the strain than the other three factors after freezing,and the following order from large to small is observed: glycerol,trehalose,sucrose,DMSO.The optimum composition of cryoproctants includes 100 g/L glycerol,100 g/L trehalose,180 g/L sucrose, and 50 g/L DMSO,which makes the livability up(to 89%.) |
| |
| Keywords: | Acidithiobacillus ferrooxidans cryoprotectants orthogonal experimental method preservation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
