基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价

观察复方苦参洗剂中君、臣、佐各药组联合抑菌性质以及协同增效作用。采用加热回流水提法提取各药组获得相应提取液,采用二倍稀释法测得相应各药组单用及联合对白色念珠菌的最低抑菌浓度(ρMIC)，采用联合抑菌指数（IFIC）判定两药组联合抑菌作用性质。结果显示，8味中药水提液的ρMIC范围为7.50 ~30.00 mg/mL,君、臣、佐各药组的 ρMIC范围为4.01 ~21.00 mg/mL，复方组ρMIC为2.22 mg/mL，黄柏+苦参、黄柏+白矾、花椒+白矾的IFIC≤0.5，黄柏+花椒、苦参+花椒、苦参+白矾3组0.5＜IFIC≤1；君药组+臣药组IFIC=0.375 0，君药组+佐药组IFIC=0.750 0，臣药组+佐药组IFIC=1.000 0。复方组及君药组的联合抑菌效果明显强于单味中药的抑菌效果，黄柏、苦参、花椒、白矾4味君药对白色念珠菌抑菌活性较强，复方苦参洗剂抑菌效果的增强与各药组抑菌作用的协同和相加有关。

Evaluation of antifungal effect of Compound Sophorae Flavescentis RadixLotion based on antifungal effect of Candida albicans in vitro

The combined antifungal properties and synergistic effects of monarch drug, ministerial drug and adjuvant drug of Compound Sophora Flavescens Radix lotion on Candida albicans in vitro were observed in this paper. The extract solution of each group of Compound Sophora Flavescens Radix lotion was obtained by heating backflow water extraction method, the minimal inhibitory concentration(ρMIC)of each group alone and in combination on Candida albicans was measured by double dilution method, using the fraction inhibitory concentration index(IFIC) to determine the combined effects of two traditional Chinese medicines (TCMs). Results showed that the ρMIC range of 8 kinds of TCM water extraction was 7.50~30.00 mg/mL, the ρMIC range of monarch drug, ministerial drug and adjuvant drug was 4.01~21.00 mg/mL, the ρMIC of compound group was 2.22 mg/mL. The IFIC of Phellodendri Chinensis Cortex+Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex+Alumen and Zanthoxyli Pericarpium+Alumen were less than or equal to 0.5, the IFIC of Phellodendri Chinensis Cortex+Zanthoxyli Pericarpium, Sophorae Flavescentis Radix+Zanthoxyli Pericarpium and Sophorae Flavescentis Radix + Alumen were greater than 0.5 and less than or equal to 1. The IFIC of monarch drug+ministerial drug was 0.375 0, the IFIC of monarch drug+adjuvant drug was 0.750 0，the IFIC of ministerial drug+adjuvant drug was 1.000 0.The study showed that the combined antifungal effect of Compound group and monarch group were stronger than that of single herbs. The 4 kinds of monarch drug of Phellodendri Chinensis Cortex, Sophorae Flavescentis Radix, Zanthoxyli Pericarpium and Alumen have strong antifungal activity against Candida albicans. The enhancement of the antifungal effect of compound Sophorae Flavescentis Radix Lotion was related to the synergy and addition of the antifungal effect of each drug group.