液相色谱-质谱联用测定不同软膏中的莫匹罗星

建立了一种液相色谱-质谱联用测定不同软膏中莫匹罗星含量的方法。色谱柱为Agilent XDB-C18（2.1 mm×50 mm，1.8 μm）；水相为 0.1 %甲酸-水，有机相为乙腈，梯度洗脱，流速为 0.3 mL·min^-1，柱温30 ℃。采用ESI 离子源正离子模式，多反应离子监测模式下选择离子对501.3→326.7、501.3→308.9和501.3→291.0进行测定。结果表明，莫匹罗星检测质量浓度线性范围为10～500 ng·mL^-1（r＝0.999 5）；检出限和定量限分别为4、10 ng·mL^-1，精密度实验的相对标准偏差（n＝6）为0.8％；低、中、高浓度的加标回收率（n=3）分别为99.8%、109.2% 和105.0% ，相对标准偏差（n＝3）分别为3.5%、1.6%和1.4%。该方法灵敏度高、专属性好、精密度高、简便、快速，可用于不同软膏中莫匹罗星的检测。

Determination of mupirocin in different ointments by HPLC-MS

A method for the determination of mupirocin in different ointments by the high performance liquid chromatography-mass spectrometry (HPLC-MS) was established. The analytical column was Agilent XDB-C18(2.1 mm × 50 mm，1.8 μm), the mobile phase was 0.1% formic acid solution and acetonitrile with gradient elution, and the flow rate was 0.3 mL·min^-1. The column temperature was set at 30 ℃. The detection was achieved in ESI positive ion mode with 501.3→326.7, 501.3→308.9 and 501.3→291.0. Results showed that the calibration curve was in a linearity over the range of 10～500 ng·mL^-1(r＝0 .999 5). The limit of detection and quantification was 4 ng·mL^-1and 10 ng·mL^-1, respectively. Precision was fine, with RSD（n=6）of 0.8％. The recoveries（n=3）of low, middle and high adding concentrations were 99.8%, 109.2% and 105.0%,and the RSD（n＝3） were 3.5%, 1.6% and 1.4%, respectively. The established method is sensitive, specific, accurate, simple and rapid, which is applicable for the determination of mupirocin in different ointments．