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榛子种质资源遗传多样性的RAPD分析
引用本文:冯斌,张希踔,解明,张开春. 榛子种质资源遗传多样性的RAPD分析[J]. 辽宁师范大学学报(自然科学版), 2007, 30(2): 216-219
作者姓名:冯斌  张希踔  解明  张开春
作者单位:辽宁师范大学生命科学学院,辽宁大连116029;辽宁省经济林研究所,辽宁大连,116031;北京市农林科学院林业果树研究所,北京,100093
摘    要:
利用RAPD分子标记技术,对来自欧洲、美国和我国北方的共48份榛子品种或品系(包括4个种及种间杂种)的遗传多样性进行了分析.从150条随机引物中筛选出24条引物,共扩增出142条清晰可重复的带,其中多态性带130条,比例为91.5%.聚类分析将48份榛子种质分为5组:欧榛、平榛各自列为1组;杂交榛分成2个组;另外一组包括滇榛和藏榛2份种质.研究结果对榛子的种间亲缘关系进行了验证,且为同种不同品种的亲缘关系提供了更为详细的信息.

关 键 词:榛子  RAPD  遗传多样性  聚类分析
文章编号:1000-1735(2007)02-0216-04
修稿时间:2006-09-05

Analysis of genetic diversity in Corylus by RAPD markers
FENG Bin,ZHANG Xi-chuo,XIE Ming,ZHANG Kai-chun. Analysis of genetic diversity in Corylus by RAPD markers[J]. Journal of Liaoning Normal University(Natural Science Edition), 2007, 30(2): 216-219
Authors:FENG Bin  ZHANG Xi-chuo  XIE Ming  ZHANG Kai-chun
Affiliation:1. College of Life Science, Liaoning Normal University, Dalian 116029,China; 2. Liaoning Economic Forest Institute, Dalian 116031 ,China;3. Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forest Science, Beijing 100093, China
Abstract:
Random amplified polymorphic DNA(RAPD) analysis was carried out to measure the genetic diversity of 48 accessions of Corylus(include 4 species),which were collected from Europe,America and Northern China.Twenty-six polymorphic primers were screened out of 150 random primers and 142 bands were distinguishable and reproducible.Among them,130 bands(91.5%) were polymorphic.The 48 accessions were divided into 5 groups according to the cluster analysis.Corylus avelana and C.heterophylla belonged to the individual group.The hybrid Corylus were divided into 2 different groups.The other group contained C.yunnanensis and C.ferox var.thibetica.Our results were consistent with the traditional taxonomy and provided more valuable information for Corylus accessions belonging to the same species.
Keywords:Corylus   RAPD   genetic diversity   cluster analysis
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