| 水稻矮化突变体差异表达cDNA片段的克隆与分析 |
| |
| 引用本文： | 王永胜,王景,李发强,刘筱斌,刘良式.水稻矮化突变体差异表达cDNA片段的克隆与分析[J].中山大学学报(自然科学版),2001,40(6):59-62. |
| |
| 作者姓名： | 王永胜 王景 李发强 刘筱斌 刘良式 |
| |
| 作者单位： | 1. 中山大学
2. 中国科学院华南植物研究所, |
| |
| 基金项目： | 教育部重点科学技术资助项目(061C615);广东省科技计划资助项目(99M01503G) |
| |
| 摘 要： | 利用抑制性消减杂交（Suppressive subraction hybridization,SSH）分离本实验室获得的一株水稻矮化突变体dwarf69与其野生型对照品系特光矮-2（Rryza sativa,TGA-2 indica）间表达有差异的cDNA片段，分别以dwarf69作为驱赶子，TGA-2为检测子，以及以dwarf69作为检测子，TGA-2为驱赶子建立了两个差异表达cNDA文库，分别代表在TGA-2和dwarf69中特异表达的cDNA，经反式Northern检测这两个文库共得到10个阳性克隆。
|
| 关 键 词： | 抑制性消减杂交 矮化突变体 反式Northern 水稻 cDNA片段 表达 克隆 |
| 文章编号： | 0529-6579(2001)06-0059-04 |
| 修稿时间： | 2001年4月2日 |
Identification of cDNA Fragments Differentially ExpressedBetween a Rice Mutant dwarf69 and Wild Type TGA_2 bySuppressive Subtraction Hybridization |
| |
| WANG Yong-sheng,WANG Jing,LI Fa-qiang,LIU Xiao-bin,LIU Liang-shi.Identification of cDNA Fragments Differentially ExpressedBetween a Rice Mutant dwarf69 and Wild Type TGA_2 bySuppressive Subtraction Hybridization[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2001,40(6):59-62. |
| |
| Authors: | WANG Yong-sheng WANG Jing LI Fa-qiang LIU Xiao-bin LIU Liang-shi |
| |
| Abstract: | The suppressive subtraction hybridization (SSH) technique was used to isolate the cDNA fragments,which showed differential expression between the rice mutant dwarf69 and wild type strain,TGA-2(Oryza sativa, indica).Two differential expressing cDNA libraries were constructed with the cDNAs of dwarf69 as driver and the cDNAs of TGA-2 as tester, vice versa. These two cDNA libraries indicated the differential expressing cDNA fragments in TGA-2 and dwarf69. By reverse Northern blots, ten positive clones were obtained. |
| |
| Keywords: | suppressive subtraction hybridization (SSH) dwarf mutant reverse Northern blots |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
