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| 基于ISSR西北地区珍珠猪毛菜(Salsola passerina)的遗传多样性(英文) | |
| 引用本文： | 高天鹏,高海宁,张勇,徐世健,安黎哲.基于ISSR西北地区珍珠猪毛菜(Salsola passerina)的遗传多样性(英文)[J].兰州大学学报(自然科学版),2009,45(2). |
| 作者姓名： | 高天鹏 高海宁 张勇 徐世健 安黎哲 |
| 作者单位： | 1. 兰州城市学院,化学与环境科学学院,兰州,730070;中国科学院,兰州化学物理研究所,兰州,730000 2. 河西学院,生命科学学院,甘肃,张掖,734000 3. 兰州大学,生命科学学院,兰州,730000 |
| 基金项目： | The Science ＆ Technology Support Project of Gansu Province，Science ＆ Technology Project of Lanzhou;Gansu Province，Science ＆Technology Base Project of Gansu Educational Bureau |
| 摘 要： | 运片JSSR分子标记的方法,对分布于中国北方7个居群的137个珍珠猪毛菜个体筛选出了15条引物,产生了196条带,其中153条为多态性条带,总的基因多态率为78.06%,多态性在种群水平上较低,平均多态率为45.60%.平均期望杂合度和Shannon信息指数在种间水平上分别为0.1759和0.2811,在居群水平上为0.125l和0.1977,种群内个体间的遗传分化较大,为70.96%,而种群间遗传分化较小,为29.04%,种群间的遗传一致度平均为0.9320.AMOVA分析得到了相似结果.基于Nei's遗传距离的UPGMA聚类分析将7个居群分为3组:德令哈、兰州和其余5个居群(中宁,乌海,民勤,金塔和安西).Mantel检验表明遗传距离与垂直地理分布显著相关(P |
| 关 键 词： | 珍珠猪毛菜 遗传多样性 ISSR分子标记 中国两北 |
Genetic diversity of Salsola passerina populations in northwestern China based on inter-simple sequence repeat(ISSR) |
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| GAO Tian-peng,GAO Hai-ning,ZHANG Yong,XU Shi-jian,AN Li-zhe.Genetic diversity of Salsola passerina populations in northwestern China based on inter-simple sequence repeat(ISSR)[J].Journal of Lanzhou University(Natural Science),2009,45(2). | |
| Authors: | GAO Tian-peng GAO Hai-ning ZHANG Yong XU Shi-jian AN Li-zhe |
| Institution: | 1;4;1.School of Chemistry and Environment Sciences;Lanzhou City University;Lanzhou 730070;China;2.School of Life Sciences;Hexi University;Zhangye 734000;Gansu;3.School of Life Sciences;Lanzhou University;Lanzhou 730000;4.Lanzhou Institute of Chemical Physics;Chinese Academy of Sciences;China |
| Abstract: | Genetic diversity and differentiation among seven populations of Salsola passerina in northwestern China were investigated using ISSR markers.15 ISSR primers gave rise to 196 discernible DNA fragments,of which 153(78.06%)were polymorphic.However,the polymorphism at the population level was relatively low,with an average of 45.60%.The mean expected heterozygosity and Shannon's information index were 0.1759 and 0.2811 at the species level,0.1251 and 0.1977 at the population level, respectively.Based on Nei's ... |
| Keywords: | Salsola passerina genetic diversity ISSR marker northwestern China |
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