| 3株致病疫霉拮抗放线菌复合发酵及其抑制机理 |
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| 引用本文： | 蒋继志,李莎,王会仙.3株致病疫霉拮抗放线菌复合发酵及其抑制机理[J].河北大学学报(自然科学版),2010,30(1). |
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| 作者姓名： | 蒋继志 李莎 王会仙 |
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| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002
2. 河北大学,图书馆,河北,保定,071002 |
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| 基金项目： | 河北省自然科学基金资助项目(C2007000191);;公益性行业(农业)科研专项(3-20) |
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| 摘 要： | 为提高拮抗放线菌控制马铃薯晚疫病的稳定性,采用涂布法和显微观察法对从土壤中筛选到的活体状态能显著抑制致病疫霉菌丝生长的3株放线菌进行了复合发酵方式及发酵条件的初步优化及抑菌机理的研究.结果表明:3株放线菌之间无明显拮抗作用;以培养种子液3 d、先接入NB8培养2 d后再接入NB11和NB12为最佳发酵方式,抑菌率最高可达97.7%,明显优于NB8(87.4%),NB11(20.0%)和NB12(28.2%)单独发酵液的抑制作用;当初始pH为自然值、28℃时,3株菌复合发酵后抑菌活性最高.复合发酵液对病原菌菌丝生长产生明显抑制作用,致使菌丝变形或孢子囊发生异常破裂.
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| 关 键 词： | 拮抗放线菌 复合发酵 抑制机理 |
Culture Condition Optimization of Three Actinomyces Mixed Fermentation Against Phytophthora infestans and Mechanism |
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| JIANG Ji-zhi,LI Sha,WANG Hui-xian.Culture Condition Optimization of Three Actinomyces Mixed Fermentation Against Phytophthora infestans and Mechanism[J].Journal of Hebei University (Natural Science Edition),2010,30(1). |
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| Authors: | JIANG Ji-zhi LI Sha WANG Hui-xian |
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| Institution: | 1.College of Life Sciences;Hebei University;Baoding 071002;China;2.Library;China |
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| Abstract: | In order to aquire the stable and more effective control for potato late blight,three actinomyces strains which had significant inhibitive function on the growth of Phytophthora infestans,isolated from soil were studied to evaluate their mixed fermentation condition.There were no obvious antagonistic among the three actinomyces strains,and the best ferment combination mode was that NB8 was inoculated at first,NB11 and NB12 was inoculated 2 days later.The inhibitory rate reached 97.7%,and higher than that of... |
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| Keywords: | actinomyces against pathogen mixed fermentation inhibitory medium |
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