| 米曲霉木聚糖酶高产菌株选育 |
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| 引用本文： | 李晨,陈宏文.米曲霉木聚糖酶高产菌株选育[J].华侨大学学报(自然科学版),2011,32(6):663-667. |
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| 作者姓名： | 李晨 陈宏文 |
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| 作者单位： | 华侨大学工业生物技术福建省高校重点实验室,福建泉州,362021 |
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| 基金项目： | 福建省自然科学基金资助项目(D0810015); 教育部留学回国人员科研启动项目(2010); 福建省高等学校新世纪优秀人才支持计划项目(07FJRC03) |
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| 摘 要： | 以米曲霉C-2为出发菌株,紫外诱变后,利用透明圈初筛,再分别以农业废弃物1％蔗渣-1％麸皮复合物、2％蔗渣、2％碱提蔗渣半纤维素和2％纤维素溶剂分馏法(CSLF)提取的蔗渣半纤维素为碳源摇瓶复筛,得到一株高产木聚糖酶的米曲霉菌株A73.结果表明:米曲霉A73在上述碳源培养基中,产木聚糖酶酶活力比出发菌株分别提高了281...
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| 关 键 词： | 木聚糖酶 米曲霉 紫外诱变 纤维素溶剂木质纤维素分馏法 半纤维素 |
Breed of Aspergillus oryzae Producing High-Yield Xylanase |
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| LI Chen,CHEN Hong-wen.Breed of Aspergillus oryzae Producing High-Yield Xylanase[J].Journal of Huaqiao University(Natural Science),2011,32(6):663-667. |
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| Authors: | LI Chen CHEN Hong-wen |
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| Institution: | LI Chen,CHEN Hong-wen(Key Laboratory of Industrial Biotechnology of Fujian Province University,Huaqiao University,Quanzhou 362021,China) |
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| Abstract: | Aspergillus oryzae C-2 was treated with ultraviolet irradiation.High-yield xylanase mutant A73 was obtained after primary transparent circle screening and secondary screening in which 4 different carbon sources-1% wheat bran-1% sugarcane bagasse,2% sugarcane bagasse,2% alkali pretreatment hemi-cellulose and 2% CSLF(cellulose solvent-based lignocellulose fractionation) pretreatment hemi-cellulose were used as fermentation substrates.The xylanase activity of Aspergillus oryzae A73 improved 281.15%,82.81%,56.0... |
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