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| 双水相萃取法富集分离地木耳中的藻蓝蛋白 | |
| 引用本文： | 甘林火. 双水相萃取法富集分离地木耳中的藻蓝蛋白[J]. 华侨大学学报(自然科学版), 2011, 32(6): 672-675 |
| 作者姓名： | 甘林火 |
| 作者单位： | 华侨大学化工学院,福建泉州,362021 |
| 基金项目： | 华侨大学科研基金资助项目(08X0211*) |
| 摘 要： | 采用聚乙二醇(PEG4000)/硫酸盐双水相体系,经一次萃取从地木耳细胞破碎液中富集分离藻蓝蛋白.分别考察萃取时间、硫酸盐种类及浓度、PEG4000浓度、溶液pH值和离子强度对双水相萃取分离地木耳中藻蓝蛋白的影响.结果表明:在萃取时间为30 min,Na2SO4的质量分数为15％,PEG4000的质量分数为12％,加入... |
| 关 键 词： | 地木耳 藻蓝蛋白 聚乙二醇 双水相体系 萃取 |
Aqueous Two-Phase Extraction for Enrichment and Separation of Phycocyanin from Nostoc |
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| GAN Lin-huo. Aqueous Two-Phase Extraction for Enrichment and Separation of Phycocyanin from Nostoc[J]. Journal of Huaqiao University(Natural Science), 2011, 32(6): 672-675 | |
| Authors: | GAN Lin-huo |
| Affiliation: | GAN Lin-huo (College of Chemical Engineering,Huaqiao University,Quanzhou 362021,China) |
| Abstract: | Enrichment and separation of phycocyanin from nostoc cell homogenates was studied under polyethylene glycol(PEG 4000)/sulphate aqueous two-phase system by one step extraction.The effects of time,type and concentration of sulphate,the concentration of PEG 4000,ionic strength and pH value on the extraction behavior were investigated respectively.The optimized extraction rate of phycocyanin from nostoc was 95.2% and reached the purity of 4.8,and the optimum conditions for extraction were extraction time of 30 ... |
| Keywords: | nostoc phycocyanin polyethylene glycol aqueous two-phase system extraction |
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