大鼠SNL神经性疼痛模型的建立

摘要:目的　建立大鼠L5和L6脊神经结扎(Spinal nerve ligation，SNL)疼痛模型并进行评价， 方法　根据大鼠痛阈值测定结果，选择90只符合要求的SD雄性大鼠进行手术操作，术后每隔1d测定大鼠后足的机械痛阈值，比较术前和术后大鼠对疼痛刺激的反应变化。 结果　术后大鼠左足的痛阈值逐渐降低，至10d 后趋于稳定，与术前有明显差异(P≤0.01)；右足的痛阈值微有升高，保持在稳定的水平内。 结论　本研究建立的大鼠SNL模型机械痛阈变化明显，稳定性好，可靠性高，可以作为大鼠一种神经病理性疼痛的模型。

Establishment of Neuropathic Pain Model by Ligated of L5 and L6 Spinal Nerve in Rats

Objective To establish the animal model of L5 and L6 spinal nerve ligation （SNL） model of neuropathic pain rats. Method According to rat pain threshold, choose 90 SD rates of male surgery operation that the left L5 and L6 spinal nerves were exposed and tightly ligated with 6 -0 black silk, postoperative measured every other day after the left hind legs of rats mechanical pain threshold, compare the preoperative and postoperative reaction to pain stimulation change in rats. Result Postoperative the left hind legs of rats pain threshold gradually reduce, to 10 days to stabilize, and the preoperative have obvious difference （P ≤ 0.01 ） ; The right hind legs of rats pain threshold were slightly elevated, remain in a stable level. Conclusion This research established SNL rats model of mechanical pain threshold changes significantly, good stability, high reliability, can be used as a kind of neuropathy pain model in rats.