不同采血方法对Wistar大鼠血常规的影响

目的探讨不同采血方法对Wistar大鼠血常规测定结果的影响。方法 40只SPF级Wistar大鼠顺序进行断尾采血、眼内眦采血和腹主动脉采血,用光电血细胞分析仪进行检测,3种采血方法的检测结果两两比较,进行配对t检验。结果断尾采血白细胞计数最高,其次为眼内眦采血,腹主动脉采血白细胞最低,约为断尾采血的1/3,3种采血途径相互之间差异都极显著(P0.01)。红细胞计数及血红蛋白指标方面,雄性大鼠3种采血途径间无显著差异(P0.05),而雌性大鼠断尾采血和眼内眦采血间差异极显著(P0.01),腹主动脉采血和其他两种采血途径间无显著差异(P0.05)。血小板计数方面,末梢血(断尾、眼内眦采血)极显著低于腹主动脉(P0.01),约为腹主动脉采血的1/2,断尾和眼内眦采血间血小板计数无显著差异(P0.05)。结论不同采血方法血常规检测结果具有较大统计学差异,应建立各自的背景数据以进行统一比较。

Influence of Different Sampling Methods on Blood Routine in Wistar Rat

Objective To compare the hematological indexes of blood samples collected from different ways in Wistar rats. Method Blood samples were collected from tail, orbital and abdominal aorta of 40 SPF Wistar rats and were detected by Nihon Kohden MEK -6318K hematology analyzer. The parameters of WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, LYM, MID and GRN from three sampling ways were analyzed and pairwise compared using paired t test. Result WBC count in the tail blood was the most, and following was the orbital blood, and WBC count in the abdominal aorta blood was the least, which was 1/3 of that in the tail blood. There was highly significant difference between the three methods （P 〈 0.01 ）. There were no significant differences between the three kinds of sampling methods of HGB and RBC count in male rats （P 〉 0. 05）. However in female rats, HGB and RBC count in the tail blood were higher than those in the orbital blood （P 〈0.01 ）. PLT count in the peripheral blood （the tail blood and the orbital blood） was less than that in the abdominal aorta blood with highly significant difference （P 〈 0.01 ）. Conclusion There were significant differences between the three methods, so each laboratory should set up own basic data for blood routine.