| 采用正交设计和响应曲面设计法优化芦苇随机扩增多态DNA体系 |
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| 引用本文： | 刘金文,沙伟,滕兆岩.采用正交设计和响应曲面设计法优化芦苇随机扩增多态DNA体系[J].广西科学,2004,11(2):148-150,160. |
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| 作者姓名： | 刘金文 沙伟 滕兆岩 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006;齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006;齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006 |
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| 摘 要： | 采用正交设计和响应曲面设计(RSD)优化适合于芦苇Phragmites australis (Cav.)Trin.ex steud]增多态DNA(RAPD)的反应体系，结果确定芦苇RAPD反应体系的最佳方案为：在PCR扩增程序，冷启动，94℃变性1min，36℃复性90s，72℃延伸2min，40个循环；72℃延伸7min，4℃保存；25μl反应体系包括模板DNA 60ng、三磷酸脱氧核苷酸(dNTP)7．5mmol、Taq酶1．5u、引物5pmol。
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| 关 键 词： | 芦苇 RAPD 正交设计 响应曲面设计 条件优化 |
| 收稿时间： | 2003/9/12 0:00:00 |
| 修稿时间： | 2004/1/29 0:00:00 |
Optimal of RAPD Realtion System on Phragmites australis by Orthogonal Design and Response Surface Design |
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| Liu Jinwen,Sha Wei and Teng Zhaoyan.Optimal of RAPD Realtion System on Phragmites australis by Orthogonal Design and Response Surface Design[J].Guangxi Sciences,2004,11(2):148-150,160. |
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| Authors: | Liu Jinwen Sha Wei and Teng Zhaoyan |
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| Institution: | The Life Sci and Engineering College, Qiqihaer Univ, Qiqihaer, Heilongjiang, 161006, China,The Life Sci and Engineering College, Qiqihaer Univ, Qiqihaer, Heilongjiang, 161006, China and The Life Sci and Engineering College, Qiqihaer Univ, Qiqihaer, Heilongjiang, 161006, China |
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| Abstract: | |
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| Keywords: | Phragmites australis RAPD orthogonal design response surface design RAPD optimalAbstract Genome DNA was extracted from the tissue of the white spot virus infected cultured shrimp Penaeus monodon by TN homogenizing DS digesting proteinase K dec |
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