| 玉米弯孢叶斑病菌ATMT转化条件优化及转GFP单孢的获得 |
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| 引用本文： | 陈茂功,林小虎,李洪杰,王晓鸣,周印富.玉米弯孢叶斑病菌ATMT转化条件优化及转GFP单孢的获得[J].河北科技师范学院学报,2011,25(3):52-57. |
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| 作者姓名： | 陈茂功 林小虎 李洪杰 王晓鸣 周印富 |
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| 作者单位： | 1. 河北科技师范学院生命科技学院,河北秦皇岛,066004
2. 中国农业科学院作物科学研究所 |
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| 基金项目： | ”十二·五”国家重大科技支撑计划项目“国家粮食丰产科技工程”(项目编号:2011BAD16B08) |
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| 摘 要： | 为了明确玉米弯孢叶斑病菌的侵入机理,对农杆菌介导法（ATMT）转化弯孢叶斑病菌的条件进行了探究。以农杆菌AGL-1为侵染菌系、pCAMDGFP作为转化载体。结果表明,玉米弯孢叶斑病菌的ATMT最佳转化条件为：分生孢子萌发12 h后,与农杆菌在25℃黑暗条件下共培养48 h,共培养介质为微孔滤膜。转化效率可达到100～1...
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| 关 键 词： | 农杆菌转化 玉米弯孢叶斑病菌 GFP |
Optimization of Factors Affecting Agrobacterium-mediated Curvularia lunata and Characterization of Transgenic GFP Spores |
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| CHEN Mao-gong,LIN Xiao-hu,LI Hong-jie,ZHOU Yin-fu.Optimization of Factors Affecting Agrobacterium-mediated Curvularia lunata and Characterization of Transgenic GFP Spores[J].Journal of Hebei Normal University of Science & Technology,2011,25(3):52-57. |
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| Authors: | CHEN Mao-gong LIN Xiao-hu LI Hong-jie ZHOU Yin-fu |
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| Institution: | CHEN Mao-gong1,LIN Xiao-hu1,LI Hong-jie2,ZHOU Yin-fu1(1 College of Life Science and Technology,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066004,2 Institute of Crop Science,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | In order to analyze the Curvularia lunata ingression mechanism,this study explored the main factors affecting Agrobacterium-mediated Curvularia lunata.The recombinant vector pCAMDGFP was transformed in this study into fresh conidia of Curvularia lunata via Agrobacterium tumefaciens（AGL-1）.The results showed that germination time of Curvularia lunata conidia was 12 h at 25 ℃;co-culturing time of the germinated conidia and bacteria was 48 h at 25 ℃;co-culturing media was millipore filter;and the transformation frequency was up to 100-110 transformants per 106 conidia.Transgenic GFP Curvularia lunata spores were also generated in this study. |
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| Keywords: | Agrobacterium transformation Curvularia lunata GFP |
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