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不同品级拟黑多刺蚁(Polyrhachis vicina)脑部雌激素相关受体基因的表达
引用本文:欧阳霞辉,李春鸣,魏锁成,侯兰新,奚耕思. 不同品级拟黑多刺蚁(Polyrhachis vicina)脑部雌激素相关受体基因的表达[J]. 陕西师范大学学报(自然科学版), 2012, 0(3): 62-66
作者姓名:欧阳霞辉  李春鸣  魏锁成  侯兰新  奚耕思
作者单位:西北民族大学生命科学与工程学院;陕西师范大学生命科学学院
基金项目:国家自然科学基金资助项目(31060350);中央高校基本科研业务费专项资金项目(ZYZ2011067)
摘    要:
采用原位杂交方法对不同品级拟黑多刺蚁(Polyrhachis vicina)脑部雌激素相关受体pvERRmRNA的表达进行了检测.结果表明,pvERRmRNA在不同品级的蕈形体、附叶、视叶、中脑、食道下神经节和球状细胞中都有表达.蚁后蕈形体和球状细胞中pvERRmRNA的阳性表达最为明显;工蚁中脑pvERR mRNA的阳性反应强度明显高于蚁后和雄蚁;pvERR mRNA在雄蚁视叶中有较强的表达.pvERR mRNA在不同品级的拟黑多刺蚁脑部的表达差异可能与它们各自在蚁群中的功能和行为有关.

关 键 词:拟黑多刺蚁  pvERR  原位杂交  昆虫脑部

Expression of estrogen related receptor gene in the brain of different caste of Polyrhachis vicina
OUYANG Xia-hui,LI Chun-ming,WEI Suo-cheng,HOU Lan-xin,XI Geng-si. Expression of estrogen related receptor gene in the brain of different caste of Polyrhachis vicina[J]. Journal of Shaanxi Normal University: Nat Sci Ed, 2012, 0(3): 62-66
Authors:OUYANG Xia-hui  LI Chun-ming  WEI Suo-cheng  HOU Lan-xin  XI Geng-si
Affiliation:1 College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,Gansu,China; 2 College of Life Science,Shaanxi Normal University,Xi′an 710062,Shaanxi,China)
Abstract:
The expression of pvERR mRNA in the brain of different caste of Polyrhachis vicina was detected by in situ hybridization method.The results showed that pvERR mRNA has broad expression pattern in the brain of different castes,which can be detected in mushroom body,accessory lobe,optic lobes,deutocerebrum,suboesophageal ganglion and kenyon cells.Strongly positive expression was detected in mushroom body and kenyon cells in brain of queen ant.Compared with queen ant,the expression degree of pvERR mRNA is stronger in the deutocerebrum of worker ant,but other parts are equivalent.Compared with queen and worker ants,the degree of pvERR expression is weaker in mushroom body,accessory lobe and suboesophageal ganglion of male ants,but stronger expression was found in optic lobes.
Keywords:Polyrhachis vicina  pvERR  in situ hybridization  insect brain
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