| 青海细叶百合鳞片诱导培养基筛选 |
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| 引用本文： | 徐丽萍,唐道城,马晶晶.青海细叶百合鳞片诱导培养基筛选[J].青海大学学报,2009,27(1). |
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| 作者姓名： | 徐丽萍 唐道城 马晶晶 |
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| 作者单位： | 徐丽萍,XU Li-ping(青海大学高原花卉研究中心,青海西宁810016;西宁市蔬菜研究所,青海西宁,810003);唐道城,马晶晶,TANG Dao-cheng,MA Jing-jing(青海大学高原花卉研究中心,青海西宁,810016)
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| 摘 要： | 选择青海省贵德县驯化3年以上的细叶百合鳞片做外植体,以MS作为基本培养基,采用2因素NAA(0、0.3、0.6、1.0 mg/L)、6-BA(0、0.5、1.0、1.5 mg/L)4水平随机区组设计,进行细叶百合鳞片不同激素浓度诱导分化培养基筛选试验。结果表明:MS+NAA0.3 mg/L+6-BA0.5 mg/L为细叶百合愈伤组织分化的最佳培养基;MS+NAA1.0mg/L+6-BA0.5mg/L可为细叶百合生根的适宜培养基。
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| 关 键 词： | 细叶百合 鳞片 诱导 培养基 |
Culture medium screening for bulb scale induction of Lilium pumilum native to Qinghai |
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| XU Li-ping,TANG Dao-cheng,MA Jing-jing.Culture medium screening for bulb scale induction of Lilium pumilum native to Qinghai[J].Journal of Qinghai University(Natural Science),2009,27(1). |
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| Authors: | XU Li-ping TANG Dao-cheng MA Jing-jing |
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| Institution: | 1.Plateau Flower Research Center of Qinghai University;Xining 810003;China;2.Institute of Vegetable of Xining;China |
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| Abstract: | Bulb scales of Lilium pumilum,which have been acclimated for at least 3 years,were used as explant and MS culture medium was used as basic medium to induce callus differentiation.A Randomized Complete Block Design with two factors and each factor had 4 levels to conduct the research.The two factors were NAA(0,0.3,0.6,1.0 mg/L) and 6-BA(0,0.5,1.0,1.5 mg/L).The results indicated that the optimal culture medium for callus initiation was MS+NAA0.3 mg/L+6-BA 0.5 mg/L.For root growth,MS+NAA1.0 mg/L+6-BA 0.5 mg/L ... |
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| Keywords: | Lilium pumilum bulb scales induction culture medium |
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