Effect of lanthanum ions on tRNAphe structure: Imino proton NMR spectroscopy |
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Authors: | Huamin Tu Yijie Wu Yansheng Yang Menglian Gong Hongjie Zhang |
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Institution: | (1) Department of Chemistry, Zhongshan University, 510275 Guangzhou, China;(2) Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China |
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Abstract: | The effect of lanthanum ions on the structural and conformational change of yeast tRNAphe was studied by1H NMR. The results suggest that the tertiary base pair (G-15)(C-48), which was located in the terminal in the augmented dihydrouridine
helix (D-helix), was markedly affected by adding La3+ and shifted 0.33 downfield. Based pair (U-8)(A-14), which is associated with a tertiary interaction, links the base of the
acceptor stem to the D-stem and anchors the elbow of the L structure, shifted 0.20 upfield. Another imino proton that may
be affected by La3+ in tRNAphe is the tertiary base pair (G-19)(C-56). The assignment of this resonance is tentative since it is located in the region of
highly overlapping resonances between 12.6 and 12.2. This base pair helps to anchor the D-loop to the TΨC loop. |
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Keywords: | lanthanum ion transfer ribonucleic acid NMR |
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