| Spontaneous Neurotransmitter Release Depends on Intracellular Rather than ER Calcium Stores in Cultured Xenopus NMJ |
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| 作者姓名： | 葛松 李如心 亓磊 何湘平 谢佐平 |
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| 作者单位： | Department of Biological Sciences and Technology Tsinghua University Beijing 100084 China，Department of Biological Sciences and Technology Tsinghua University Beijing 100084 China，Department of Biological Sciences and Technology Tsinghua University Beijing 100084 China，Department of Biological Sciences and Technology Tsinghua University Beijing 100084 China，Department of Biological Sciences and Technology Tsinghua University Beijing 100084 China |
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| 基金项目： | Supported by the Natural Science Foundation of Beijing (No. 5052015) |
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| 摘 要： | Introduction During development, many cell types exhibit sponta-neous neurotransmitter release, with synaptic transmis-sions crucial for normal nervous system activity. Syn-aptic transmissions are initiated when an action poten-tial triggers the neurotran…
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| 关 键 词： | 神经递质 钙离子 内质网状组织 神经传导 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-212005-12-27 |
Spontaneous Neurotransmitter Release Depends on Intracellular Rather than ER Calcium Stores in Cultured Xenopus NMJ |
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| GE Song,LI Ruxin,QI Lei,HE Xiangping,XIE Zuoping.Spontaneous Neurotransmitter Release Depends on Intracellular Rather than ER Calcium Stores in Cultured Xenopus NMJ[J].Tsinghua Science and Technology,2006,11(4):440-446. |
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| Authors: | GE Song LI Ruxin QI Lei HE Xiangping XIE Zuoping |
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| Institution: | Department of Biological Sciences and Technology, Tsinghua University, Beijing 100084, China |
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| Abstract: | Calcium ions are important in many vital neuron processes, including spontaneous neurotransmitter release. Extracellular calcium has long been known to be related to spontaneous neurotransmitter release, but the detailed mechanism for the effect of intracellular Ca2+ on synaptic release has not yet been understood. In this research, 1,2-bis-(o-aminophenoxy)-ethane-N, N, N’, N’-tetraacetic acid tetraacetoxy-methyl ester (BAPTA-AM) was used to combine with cytosolic free Ca2+ in a calcium free medium of cultured Xenopus neuromuscular junctions (NMJ). The spontaneous synaptic current (SSC) frequency was obviously reduced. Then, drugs were applied to interrupt and activate the Ca2+ release channels in the endoplasmic reticulum (ER) membrane, but the SSC frequency was not affected. The results show that spontaneous neurotransmitter release depends on intracellular rather than ER calcium in cultured Xenopus NMJ without extracellular calcium. |
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| Keywords: | spontaneous neurotransmitter extracellular Ca2 intracellular Ca2 endoplasmic reticulum |
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