| Obtaining High Pest-resistant Tobacco Plants Carrying B.t. insecticidal Gene |
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| 作者姓名： | Shi Chunlin Zhu zhen Chen Lei Xu Honglin Xiao Guifang Feng Pingzhang Illimar Altosaar |
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| 作者单位： | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R.China,Institute of genetics, Chinese Academy of Sciences, Beijing 100101, P.R.China,Department of Biochemistry, University of Ottawa, Ottawa, Canada |
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| 基金项目： | 国家高技术研究发展计划(863计划)，中国科学院资助项目，，，， |
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| 摘 要： | To increase the expression level of CryIA(c) gene in transgenic plants, a plant expression vector pBinMoBc carrying the CryIA(c) gene under control of chimeric OM promoter and Ω factor was constructed. As a control, pBinoBc carrying the CryIA(c) gene with the CaMV 35S promoter was also constructed. The vectors were transferred into tobacco plants respectively via Agrobacterium-mediated transformation. ELISA assay showed that the expression level of the CryIA(c) gene in pBinMoBc transgenic tobacco plants was 2.44-times that in pBinoBc transgenic tobacco plants, and it could be up to 0.255% of total soluble proteins. Bioassay showed that pBinMoBc transgenic tobacco plants had more notable insecticidal effect than pBinoBc transgenic tobacco plants. The above results showed that the chimeric OM promoter was a stronger promoter than CaMV 35S promoter that was widely used in plant genetic engineering, and this is very useful in pest-resistant plant genetic engineering.
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| 关 键 词： | Transgenic plant Insecticidal gene |
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