微波辅助萃取-高效液相色谱法测定苦丁茶中熊果酸的含量

采用微波辅助萃取—高效液相色谱法测定了苦丁茶中熊果酸的含量.苦丁茶粉碎后以乙醇为溶剂,微波高火提取4 min,采用高效液相色谱法（HPLC）测定熊果酸的含量.色谱柱为InertsilC18柱（5μm,4.6×150 mm）;流动相为甲醇/水（体积比90∶10）,流速为0.8mL/min,柱温40℃,检测波长为210nm.结果表明熊果酸在0.0996~0.4980mg/mL范围内线性关系良好,平均回收率为100.8%,RSD=1.12%,苦丁茶中熊果酸的含量为14.55 mg/g.

Determination of ursolic acid in kudingcha by microwave extraction-HPLC

Using microwave extraction—High Performance Liquid Chromatographic（HPLC） method to detecte the content of ursolic acid in kudingcha.The kudingcha were crushed and were extracted by microwave-assisted.The microwave extraction method as follows： ethanol as solvent,microwave power of middle fire and extraction time of 4 minute.The analysis was carried out on a Inertsil C18 column（5 μm,4.6×150 mm）with a mobile phase of methanol/ H2O（V∶V=90∶10）,at flow rate of 0.8 ml/min.The column temperature was set at 40℃ and the wave—length was 210 nm.The result indicated that the linear ranges was 0.099 6～0.498 0 mg/mL,the average recovery rate was 100.8% and the RSD was 1.12%（n=5）.The content of ursolic acid in kudingcha was 14.55 mg/g.