Marrow mesenchymal stem cells transduced with TPO/FL genes as support for ex vivo expansion of hematopoietic stem/progenitor cells |
| |
| Authors: | C-g Xie J-f Wang Y Xiang B-b Jia L-y Qiu L-j Wang G-z Wang G-p Huang |
| |
| Institution: | (1) Institute of Stem Cell Biology, College of Life Sciences, Zhejiang University, 232 Wen San Road, Hangzhou, 310012, P. R. China;(2) Institute of Life Science, Jiangsu University, Zhenjiang, 212013, P. R. China |
| |
| Abstract: | A new marrow-derived mesenchymal stem cell (hMSC) line that could support expansion of hematopoietic stem/progenitor cells
(HSPCs) was developed. Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. CD34+
cells from cord blood were expanded with primary hMSCs or transduced hMSCs. The expansion of total nucleated cells, CD34+
cells and mixed colonies containing erythroid and myeloid cells and megakaryocytes for 2 weeks coculture with transduced hMSCs
was remarkably increased. The outputs of long-term culture-initiating cells for 2 and 4 weeks coculture with transduced hMSCs
were also largely increased. The expansion rates of HSPCs with transduced hMSCs were unchanged for 6 weeks. In contrast, the
expansion rates of HSPCs with primary hMSCs declined drastically through 6 weeks. SCID-repopulating cell expansion with transduced
hMSCs for 4 weeks was significantly higher than that of uncultured CD34+ cells and HSPCs expanded with primary hMSCs.
Received 21 June 2005; received after revision 30 July 2005; accepted 24 August 2005 |
| |
| Keywords: | Mesenchymal stem cell transduction thrombopoietin Flt-3 ligand hematopoietic stem/progenitor cell expansion |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
