自发性先天性白内障大鼠皮肤移植及生化位点的研究

目的 探讨近交系同系异体自发性先天性白内障(TSC)大鼠皮肤移植反应的影响以及生化标记基因位点的测定.方法 选用TSC大鼠10只,6～8周龄,体质量170～200 g,雌雄各半,采用背-背皮肤移植法进行自体和异体皮肤移植,一周后拆除包扎,观察皮片生长情况.取基础群F29代的同一窝群TSC大鼠6只,6～8周龄,体质量20...

Research of Skin Transplantation and Biochemical Marker Genes in Spontaneous Marker Genes in Spontaneous Congenital Cararact Rats

Abstract: Objective To investigate the anti-rejection effect of xenogenic skin transplantation and biochemical marker genes in congenital cataract rats ( TSC) . Method Ten male TSC rats (170-200 g) were chosen for tergal skin transplantion of the autologous and allogeneic. One week later, bandage was removed and skin groeth was observed. We chose 6 TSC rats (200-220 g) from the same nest of basic group F29 generation, half male and half female, aged from 6 - 8 weeks. Tests were measured according to Chinese national standards GB / 14923—2010( laboratory animal-genetic quality control of mammalian laboratory animals) and GB / T 14927. 1—2008 ( laboratoryanimals-method for biochemical markers of inbred mice and rats) . Result The skin graft survival with conclusious observation of 100 days from TSC rats. The marker loci were Akp1 a, Cs1 a, Es1a, Es3 a, Es4 b, Es6 b, Es8 b, Es9 c, Es10 a, Hbb b and Alp1 b, respectively. Referring to the biochemical genetic profile of the inbred rat strains commonly used at present, we did not find exactly the same biochemical markers with our TSC rats. Our TSC rats have unique genetic profiles. Conclusion There was no rejection after skin transplantation, which indicated that the histocompatibility gene of inbred TSC rats was consistent. The result of 11 biochemical markerssuch as esterase-1 were homozygous, which met the quality standards of inbred lines.