| 大孔吸附树脂纯化新疆两色金鸡菊总皂苷 |
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| 引用本文： | 姚新成,张婷,唐辉,陈文.大孔吸附树脂纯化新疆两色金鸡菊总皂苷[J].河北大学学报(自然科学版),2016,36(3):242. |
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| 作者姓名： | 姚新成 张婷 唐辉 陈文 |
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| 基金项目： | 新疆生产兵团重点领域科技攻关项目(2014BA031);石河子大学高层次人才科研启动项目(RCZX201328);国家“重大新药创制”科技重大专项(2011ZX09401-007) |
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| 摘 要： | 利用大孔吸附树脂纯化新疆两色金鸡菊总皂苷.采用分光光度法测定总皂苷含量,以静态吸附量和解吸率、动态吸附量和解吸率为指标,选择合适的大孔吸附树脂和纯化条件.D-101型大孔吸附树脂对新疆两色金鸡菊总皂苷具有较强的吸附性能和较好的解吸能力.两色金鸡提取液经D-101型大孔吸附树脂处理,可获得质量分数为(40.53±0.81)%(n=3)的总皂苷部位.D-101型大孔吸附树脂可有效地纯化新疆两色金鸡菊总皂苷.
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| 关 键 词： | 两色金鸡菊 总皂苷 大孔吸附树脂 |
| 收稿时间： | 2015-08-20 |
Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin |
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| YAO Xincheng,ZHANG Ting,TANG Hui,CHEN Wen.Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin[J].Journal of Hebei University (Natural Science Edition),2016,36(3):242. |
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| Authors: | YAO Xincheng ZHANG Ting TANG Hui CHEN Wen |
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| Institution: | 1.School of Pharmacy, Shihezi University, Shihezi 832000, China; 2.Ministry of Education KeyLaboratory of Xinjiang Endemic Phytomedicine Resources, Shihezi University, Shihezi 832000, China |
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| Abstract: | To investigate the purification process of total saponins from Coreopsis tinctoria Nutt.using macroporous adsorptive resin.The assay of total saponins was determined by UV-Vis spectrophotometry,and the resins were selected using the parameters,such as static adsorption capacity,desorption ratio,dynamic adsorption capacity,and as well as dynamic desorption ratio.The D-101 macroporous resin is suitable for purification of total saponins from Coreopsis tinctoria Nutt.according to its adsorption and desorption ability for total saponins.The total saponins content is(40.53±0.81)%(n=3)in total solids through D-101 macroporous resin purification.The D-101 macroporous resin can be used for purification of total saponins obtained from Coreopsis tinctoria Nutt.in Xinjiang. |
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| Keywords: | Coreopsis tinctoria Nutt total saponins macroporous adsorptive resin |
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