小麦与高冰草体细胞杂种F5代麦谷蛋白及SDS沉降值分析

通过对普通小麦与高冰草体细胞杂种F5代 175个株系的麦谷蛋白及SDS沉降值分析 ,发现杂种F5代有 10种不同的麦谷蛋白组成 ,并出现了 1Ax1,1Ax2 ,1Bx13,1By16 ,1By8,1Dx5等 6种不存在于亲本小麦中的高分子量麦谷蛋白亚基及 1Bx13 1By16 ,1Bx7 1By8,1Dx5 1Dy12等 3种新亚基组合 ,部分杂种中出现了高冰草的麦谷蛋白特征谱带 ;杂种株系的SDS沉降值表现出较大的差异 ,其中一些杂种的SDS沉降值明显高于亲本普通小麦 .结果表明 ,通过体细胞杂交有可能将野生禾草的优良性状引入普通小麦 ,创造出多种不同组成及组合的新种质 ,为小麦品质育种开辟新途径

Glutenin and SDS sedimentation analysis of the F5 somatic hybrids between Triticum aestivum L and Agropyron elongatum

Glutenins of the 175 F 5 plant lines originated from Triticum aestivum L ( ) Agropyron elongatum, together with SDS sedimentation values of some of these plants were analyzed in the present work. The hybrids showed 10 different electrophoretic patterns of high molecular weight glutenin subunits (HMW-GS) composition. Six subunits, such as 1Ax1,1 Ax2 *,1Bx13,1By16,1By8 and 1Dx5 with 1By8 and 1By16 having the same mobility with those from A. elongatum, and three allelic combinations (1Bx13 1By16,1Bx7 1By8 and 1Dx5 1Dy12) occurred in the hybrids, which did not exist in the parent common wheat Jinan177. SDS sedimentation values of hybrid plant lines varied in a broad range. Some hybrids showed a distinctly higher SDS sedimentation than Jinan177. The results above indicated we can transfer the good-quality character of the wild plants into common wheat and create germplasms with different subunits and subunit combinations through somatic hybridization. It opens a new way for wheat quality breeding.