| 15-KETE对大鼠肺动脉平滑肌细胞Kv1.5、Kv2.1表达的影响 |
| |
| 引用本文： | 郭守利,孙艳,张一飞,朱大岭.15-KETE对大鼠肺动脉平滑肌细胞Kv1.5、Kv2.1表达的影响[J].实验动物科学,2013(5):9-14. |
| |
| 作者姓名： | 郭守利 孙艳 张一飞 朱大岭 |
| |
| 作者单位： | [1]哈尔滨医科大学第二临床医学院实验动物中心,哈尔滨150086 [2]哈尔滨医科大学公共卫生学院职业卫生与职业医学教研室,哈尔滨150086 [3]哈尔滨医科大学第二临床医学院药学部,哈尔滨150086 [4]哈尔滨医科大学院药学院,哈尔滨150086 |
| |
| 基金项目： | 黑龙江省科技攻关项目（No.PC09S03） |
| |
| 摘 要： | 目的 从细胞分子水平研究电压门控型钾通道亚型Kv1.5、Kv2.1在15-酮基二十碳四烯酸（15-ketoeicosatetretraenoic acid,15-KETE）致大鼠肺动脉收缩过程中的作用.方法 通过酶法分离、培养SD大鼠肺动脉平滑肌细胞（pulmonary arterial smooth cells,PASMCs）,使用RT-PCR和Western blot技术观察15-KETE对大鼠PASMCs上Kv1.5、Kv2.1通道表达的影响.结果 （1）15-KETE下调大鼠PASMCs膜上Kv1.5通道mRNA及蛋白质的表达;（2） 15-KETE下调大鼠PASMCs膜上Kv2.1通道mRNA及蛋白质的表达.结论 Kv1.5、Kv2.1通道参与由15-KETE引起的缺氧肺动脉收缩（hypoxic pulmonary vasoconstriction,HPV）.
|
| 关 键 词： | 15-酮基二十碳四烯酸 缺氧肺动脉收缩 电压门控性K＋通道亚型 肺动脉平滑肌细胞 |
The Effect of 15-KETE on the Protein and mRNA Expression of Kv1.5,Kv2.1 in Rat Pulmonary Arterial Smooth Cells |
| |
| GUO Shou-li,SUN Yan,ZHANG Yi-fei,ZHU Da-ling.The Effect of 15-KETE on the Protein and mRNA Expression of Kv1.5,Kv2.1 in Rat Pulmonary Arterial Smooth Cells[J].Shiyan Dongwu Kexue,2013(5):9-14. |
| |
| Authors: | GUO Shou-li SUN Yan ZHANG Yi-fei ZHU Da-ling |
| |
| Institution: | 1.Laboroary Animal Center of the Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China; 2.Department of Occupational health and occupational medicine, School of Public Health, Harbin Medical University, Harbin 150086, China; 3.The Second Affiliated Hospital, the department of Pharmacy, Harbin Medical University, Harbin 150086, China; 4.Colledge of Pharmacy,Harbin Medical University, Harbin 150086, China;) |
| |
| Abstract: | Objective To observe the effect of Kv1.5、Kv2.1 channels in rat pulmonary arterial smooth cells on the process of pulmonary vasoconstriction induced by 15-KETE.Method Western blot and PCR were used to identify the effect of 15-KETE on expression of Kv1.5、Kv2.1 channels in cultured rat pulmonary arterial smooth muscle cells （PASMCs）.Result （1） The expressions of Kv1.5 mRNA and protein in rat PASMCs were significantly down-regulated by 15-KETE.（2）Theexpressions of Kv2.1 mRNA and protein in rat PASMCs were significantly down-regulated by 15-KETE.Conclusion Kv1.5、Kv2.1 channels are involved in vasoconstriction induced by 15-KETE in rat PA. |
| |
| Keywords: | 15-ketoeicosatetretraenoic acid hypoxic pulmonary vasoconstriction voltage-gated potassium channel subtype pulmonary arterial smooth cells |
| 本文献已被 维普 等数据库收录！ |
|
