| 猕猴B病毒妒和gC合成基因重组杆状病毒质粒的构建 |
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| 引用本文： | 严翔,曹玉华,刘慧芳,曾林,隋丽华,崔晓霞,孙兆增,何剑斌.猕猴B病毒妒和gC合成基因重组杆状病毒质粒的构建[J].实验动物科学,2013(6):6-8,12. |
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| 作者姓名： | 严翔 曹玉华 刘慧芳 曾林 隋丽华 崔晓霞 孙兆增 何剑斌 |
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| 作者单位： | [1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]军事医学科学院实验动物中心,北京100071 [3]塔里木大学生命科学院,阿拉尔84330 [4]青岛农业大学动物科技学院,青岛266109 |
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| 基金项目： | 十二五重大专项“高致病病原动物模型研究”（No.2012ZX10004502） |
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| 摘 要： | 目的构建猕猴B病毒gB和gc合成基因的杆状病毒重组质粒（rBacmid）。方法根据本室已经合成的猕猴B病毒gB基因和gc基因，利用BamHI和XhoI特异性酶切，将其亚克隆于昆虫杆状病毒的pFastBacHTA转座载体。将该阳性转座载体转化到DHlobac感受态细胞，从中筛选出阳性重组细菌，并提取重组质粒。结果获得了重组质粒bacmid—gB和bacmid—gC。结论该研究为猕猴B病毒gB和gc蛋白在杆状病毒系统内的表达奠定了基础。
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| 关 键 词： | 猕猴B病毒 gB基因 gC基因 杆状病毒 |
Construction of the Baculovirus Recombinant Bacmid of Synthetic Macaque B Virus gB and gC Genes |
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| YAN Xiang,CAO Yu-hua,＇,LIU Hui-fang,ZENG Lin,SUI Li-hua,CUI Xiao-xiaz,SUN Zhao-zeng,HE Jian-bin.Construction of the Baculovirus Recombinant Bacmid of Synthetic Macaque B Virus gB and gC Genes[J].Shiyan Dongwu Kexue,2013(6):6-8,12. |
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| Authors: | YAN Xiang CAO Yu-hua ＇ LIU Hui-fang ZENG Lin SUI Li-hua CUI Xiao-xiaz SUN Zhao-zeng HE Jian-bin |
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| Institution: | l ( 1. College of Veterinary and Animal Science, Shenyang Agricultural University, Shenyang 110161, China) (2. Academy of Military Medical Sciences Laboratory Animal Centre, Beijing 100071, Chlna ) ( 3. Tarum University college of life seienee,Xinjiang Alar 843300, China) (4. Qingdao Agricultural University college of animal science and technology, Qingdao 266109, China) |
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| Abstract: | Objective To obtain baculovirus recombinant bacmids of synthetic macaque B virus gB and gC genes. Method The gene fragments of synthetic gB and gC were cloned in the transposition plasmid pFastBac-HTA. The positive plasmids were transformed to the competent cells of DH10bac. The recombinant bacmids were selected by PCR. Result The recombinant baemids of gB and gC genes were constructed successfully. Conclusion Method of expression of gB and gC genes in the baculovirus expression system is established. |
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| Keywords: | macaque B virus gB gene gC gene baculovirus |
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