| Fenton反应产生的羟基自由基及其清除的分光光度法测定 |
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| 引用本文： | 宋艳,邹洪,董瑞.Fenton反应产生的羟基自由基及其清除的分光光度法测定[J].北京教育学院学报(自然科学版),2007,2(1):1-2,25. |
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| 作者姓名： | 宋艳 邹洪 董瑞 |
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| 作者单位： | 首都师范大学化学系，首都师范大学化学系，广州冷机股份有限公司 北京100037，北京100037，广州，510470 |
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| 摘 要： | Fenton反应产生的羟基自由基氧化二甲亚砜(DMSO)生成的甲醛与2,4-二硝基苯肼(DNPH)反应在碱性条件下生成稳定的酒红色腙类物质,其最大吸收波长为390nm,光度法测定其含量可间接测定·OH的生成量。拟定的方法测定了数种清除剂的IC50。该方法简便可靠,可用于羟基自由基清除剂的筛选.
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| 关 键 词： | 羟基自由基 清除剂 2 4-二硝基苯肼 分光光度法 |
Determination of Hydroxyl Radical and Its Scavenging by Spectrophotometry Method |
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| Song Yan,Zou Hong,Dong Rui.Determination of Hydroxyl Radical and Its Scavenging by Spectrophotometry Method[J].Journal of Beijing Institute of Education,2007,2(1):1-2,25. |
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| Authors: | Song Yan Zou Hong Dong Rui |
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| Institution: | Song Yan1,Zou Hong1,Dong Rui2 |
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| Abstract: | A new spectrophotometry method for determination of hydroxyl radical was proposed. Hydroxyl radicals, generated by Fenton reaction, attacks dimethyl sulfoxide to form formaldehyde, which then reacts with 2,4-dinitrophenylhydrazine to form stable red hydrazine that exhibits an absorption maximum at 390 nm. The quantitative analysis of hydroxyl radical can be done through the determination of the absorption. This method is simple and can be employed for the researching of the hydroxyl radical scavengers. |
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| Keywords: | hydroxyl adical scavenger 2 4-Dinitrophenylhydrazine spectrophotometry |
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