| 假丝酵母(Candida antarctic WSH112)生产生物表面活性剂及降解正构烷烃的研究 |
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| 引用本文： | 华兆哲,陈坚,朱文昌,徐星和,伦世仪,王晓蓉,孙昊,吴重华.假丝酵母(Candida antarctic WSH112)生产生物表面活性剂及降解正构烷烃的研究[J].南京大学学报(自然科学版),1998(2). |
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| 作者姓名： | 华兆哲 陈坚 朱文昌 徐星和 伦世仪 王晓蓉 孙昊 吴重华 |
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| 作者单位： | 无锡轻工大学生物工程学院环境生物技术研究室，南京大学污染控制与资源化国家重点实验室 |
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| 基金项目： | 南京大学污染控制与资源化研究国家重点实验室开放基金 |
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| 摘 要： | 研究了假丝酵母ＷＨＳ１１２以豆油为唯一碳源生产生物表面活性剂甘露糖赤藓糖醇脂（ＭＥＬ）的摇瓶发酵条件，得到的最适条件分别为接种量５％～８％（ｖ／ｖ），氮源为０．２％ＮａＮＯ３，温度为２５℃，２５０ｍｌ三角瓶装液量为３０ｍｌ，初始ｐＨ６．０；通过逐步增大正构烷烃浓度的方法进行了实验室内的菌种诱导改良工作，并通过初步研究发现，该菌种在产生生物表面活性剂的同时，对正构烷烃具有较好的降解能力（＞９０％）。
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| 关 键 词： | 生物表面活性剂，正构烷烃，CandidaantarcticWSH112 |
STUDY ON THE PRODUCTION OF BIOSURFACTANT AND BIODEGRADATION OF N ALKANES BY CANDIDA ANTARCTIC |
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| Hua Zhaozhe,Chen Jian,Zhu Wenchang,Xu Xinghe,Lun Shiyi,Wang Xiaorong,Sun Hao,Wu Chonghua.STUDY ON THE PRODUCTION OF BIOSURFACTANT AND BIODEGRADATION OF N ALKANES BY CANDIDA ANTARCTIC[J].Journal of Nanjing University: Nat Sci Ed,1998(2). |
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| Authors: | Hua Zhaozhe Chen Jian Zhu Wenchang Xu Xinghe Lun Shiyi Wang Xiaorong Sun Hao Wu Chonghua |
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| Abstract: | The shake flask ferentation conditions(including inoculation amount,Nitrogen sources,temperature,DO and pH) of Mannosylerythritol Lipids(MELs), a kind of biosurfactant produced by Candida antarctic WSH112 from the unique carbon source(soybean oil),were studied.By means of gradually increasing the concentration of n Alkane the strain was induced to assimilate n Alkane,and it showed that the strain had a fair ability for n Alkane biodegradation as well as biosurfactant production. |
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| Keywords: | Biosurfactant n Alkane Candida antarctic WSH112 |
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