| Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry |
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| 作者姓名： | 陈慕雁 Yang Hongsheng Shi Fangfang |
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| 作者单位： | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,P.R.China [2]Graduate University, Chinese Academy of Sciences, Beijing 100049, P.R.China |
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| 摘 要： | Mixtures of hemolymph from Chlamys farreri with three different anticoagulant solutions were incu-bated for an hour in vitro, then the ultrastructural alterations of hemocytes were observed, and the aggre-gation rate was analyzed by using transmission electron microscropy and flow cytometry respectively. The results showed that Formula 3 （glucose 20.8 g L^-1; EDTA 20mM; sodium chloride 20 g L^-1; Tris-HCI 0.05M;pH 7.4）was the desirable anticoagulant solution for C. farreri hemocytes. Further phagocytosis assay showed that no obvious negative effect was given to the hemocyte phagocytic activity when using Formula 3 as the anticoagulant solution.
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| 关 键 词： | 抗凝血剂 血淋巴 血细胞计数 血液研究 |
Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry |
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| Chen Muyan,Yang Hongsheng,Shi Fangfang.Selection of anticoagulant solution to the hemolymph of Chlamys farreri by transmission electron microscropy and flow cytometry[J].High Technology Letters,2007,13(2):221-224. |
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| Authors: | Chen Muyan Yang Hongsheng Shi Fangfang |
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| Abstract: | Mixtures of hemolymph from Chlamys farreri with three different anticoagulant solutions were incubated for an hour in vitro , then the ultrastructural alterations of hemocytes were observed , and the aggregation rate was analyzed by using transmission electron microscropy and flow cytometry respectively. The results showed that Formula 3 (glucose 20. 8 g L-1; EDTA 20mM ; sodium chloride 20 g L-1 ; Tris-HCl 0.05M;pH 7. 4) was the desirable anticoagulant solution for C . Farreri hemocytes. Further phagocytosis assay showed that no obvious negative effect was given to the hemocyte phagocytic activity when using Formula 3 as the anticoagulant solution. |
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| Keywords: | hemolymph anticoagulant solution TEM flow cytometry Chlamys farreri |
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