HMGB1对心肌成纤维细胞增殖和Ⅰ、Ⅲ胶原蛋白分泌的影响及意义

摘要：目的：通过观察HMGB1（ High mobility group protein-B1）对新生SD（Sprague-Dawley）大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响，探讨HMGB1在心梗后心脏重构过程中的作用。方法：以培养的新生SD（Sprague-Dawley）大鼠CFs为实验对象，分别给予0(对照组)、0.01、0.1、和1 mg/L 浓度的HMGB1进行干预，6、12、18、24和48小时后进行四氮唑盐(MTT)比色法检测CFs增殖水平，采用荧光实时定量PCR法检测Ⅰ、Ⅲ型胶原蛋白mRNA表达。结果：1.与对照组相比，48小时后，不同浓度的HMGB1组新生SD大鼠CFs增殖水平和Ⅰ、Ⅲ型胶原mRNA的表达水平均较对照组有所增高(p<0.05)。2.HMGB1浓度为0.1mg/L时CFs增殖水平和I、Ⅲ型胶原mRNA表达水平较其他处理组均升高(p<0.05)。结论：HMGB1能促进SD大鼠CFs增殖及Ⅰ、Ⅲ型胶原蛋白mRNA的表达，其作用显示在低水平时可能呈浓度依赖性，并且浓度为0.1mg/L时效应最明显，结果显示HMGB1参与了心梗后的心脏重构过程并发挥了重要作用。

Effects of HMGB1 on proliferation, collagen types I and III mRNA expression in cardiac fibroblasts

To investigate the effects of HMGB1(High mobility group protein-B1) on the proliferation and synthesis of collagen in cultured rat cardiac fibroblasts and evaluate its potential role in the process of cardiac remodeling after myocardial infarction Neonatal Sprague-Dawley rat cardiac fibroblasts were isolated,cultured and divided into 4 groups.They were treated in vitro which 0(control group),0.01,0.1 and 1 mg/L HMGB1 respectively.After 6,12,18,24 and 48 hours of treatment of HMGB1,the proliferation was dete...