| 白灵侧耳木质素酶、纤维素酶高活性菌株的诱变选育 |
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| 引用本文： | 张渊,王谦,张筱梅,张焕英.白灵侧耳木质素酶、纤维素酶高活性菌株的诱变选育[J].河北大学学报(自然科学版),2005,25(6):654-658. |
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| 作者姓名： | 张渊 王谦 张筱梅 张焕英 |
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| 作者单位： | 1.保定师范专科学校,生物系,河北,保定,071051； 2.河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省科技厅资助项目(022201504D) |
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| 摘 要： | 通过对白灵侧耳进行原生质体制备和紫外线诱变处理,筛选出1株木质素酶、纤维素酶高产菌株B203,与出发菌株相比, 羧甲基纤维素酶活力在营养生长期和子实体形成期分别是出发菌株的2.2倍和2.47倍, 滤纸酶活力分别是出发菌株的1.75倍和1.68倍,漆酶、愈创木酚氧化酶和邻苯二酚氧化酶活力在营养生长期分别是出发菌株的1.54倍、1.21倍和1.25倍;在现蕾期分别是出发菌株的1.49倍、1.81倍和1.15倍,对木质素、纤维素的降解率分别比出发菌株提高了6.87%和5.65%.
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| 关 键 词： | 白灵侧耳 纤维素酶 木质素酶 原生质体诱变 |
| 文章编号： | 1000-1565(2005)06-0654-05 |
| 修稿时间： | 2004年12月24 |
Screening of Pleurotus nebrodensis Strain Producing High Activity of Lignocellulolytic Enzymes |
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| ZHANG Yuan,WANG Qian,ZHANG Xiao-mei,ZHANG Huan-ying.Screening of Pleurotus nebrodensis Strain Producing High Activity of Lignocellulolytic Enzymes[J].Journal of Hebei University (Natural Science Edition),2005,25(6):654-658. |
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| Authors: | ZHANG Yuan WANG Qian ZHANG Xiao-mei ZHANG Huan-ying |
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| Institution: | ZHANG Yuan~1,WANG Qian~2,ZHANG Xiao-mei~1,ZHANG Huan-ying~1 |
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| Abstract: | By using protoplast regeneration and UV mutation techniques a new strain B203 of Pleurotus nebrodensis was selected.The new strain showed higher efficiency for degradation of lignin and cellulose than the original.Compared with the original strain,the activities of CMCase and FPAase of B203 were 2.2 times and 1.7 times higher at mycelial stage,2.47times and 1.68 times higher at fruitbody stage;the activities of laccase,guaiacol oxidase and polyphenol oxidase were 1.54 times,1.21 times and 1.25 times higher at mycelial stae,1.49 times,1.81 times and 1.15 times higher at fruitbody stage,the degradation rates of lignin and cellulose were increased by 6.87% and 5.65% respectively. |
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| Keywords: | Pleurotus nebrodensis cellulose enzyme lignose enzyme protoplast induced mutation |
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