| 当归多糖APS-bII的结构特征及体外抗肿瘤作用 |
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| 引用本文： | 陈曦.当归多糖APS-bII的结构特征及体外抗肿瘤作用[J].科学技术与工程,2010,10(8). |
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| 作者姓名： | 陈曦 |
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| 作者单位： | 第四军医大学药学系药理学教研室,西安,710032 |
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| 基金项目： | 国家自然科学基金(30701081)资助 |
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| 摘 要： | 从当归(Angelica sinensis(Oliv)Diels)中提取粗多糖,经阴离子交换纤维素柱和Sephadex G-100凝胶柱色谱分离纯化,得白色粉末状多糖APS-bII。采用高效尺寸排阻色谱、红外光谱、高效液相色谱等方法进行了结构的初步分析;采用四甲基偶氮唑蓝比色法观察APS-bII对多种肿瘤细胞的生长抑制作用。结果表明APS-bII为均一组分,分子量为1.3×104Da,由葡萄糖-半乳糖-木糖-阿拉伯糖(8.4:2.7:1.8:1.0)构成,是首次从当归中分离出的杂多糖。APS-bII对多种肿瘤细胞的增殖有抑制作用。
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| 关 键 词： | 当归 多糖 MTT法 抗肿瘤 |
| 收稿时间： | 12/7/2009 5:45:47 PM |
| 修稿时间： | 12/7/2009 5:45:47 PM |
Physicochemical Properties and Anti-tumor Activities in vitro of Angelica sinensis Polysaccharide APS-bII |
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| chenxi.Physicochemical Properties and Anti-tumor Activities in vitro of Angelica sinensis Polysaccharide APS-bII[J].Science Technology and Engineering,2010,10(8). |
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| Authors: | chenxi |
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| Institution: | CHEN Xi,CAO Wei,SUN Yang,MEI Qi-bing |
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| Abstract: | The polysaccharide APS-bII was isolated from Angelica sinensis(Oliv.) Diels through water extraction, deprotein, ethanol precipitation, and was further purified by DEAE-Sephadex A-25 and Sephadex G-100 column chromatography.The molecular weight was carried out with high-performance size exclusion chromatography (HPSEC).Sugar composition analysis and infrared spectrum were used to determine the structural features.The HPSEC result showed the APS-bII was a single homogeneous component and its molecular weight... |
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| Keywords: | Angelica sinensis polysaccharide MTT method anti-tumor |
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