| 西红花愈伤组织的诱导与花柱愈伤组织的继代、悬浮培养 |
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| 引用本文： | 赵军,徐莺,颜钫,唐琳,陈放.西红花愈伤组织的诱导与花柱愈伤组织的继代、悬浮培养[J].四川大学学报(自然科学版),2001,38(3):421-424. |
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| 作者姓名： | 赵军 徐莺 颜钫 唐琳 陈放 |
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| 作者单位： | 四川大学生命科学学院 |
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| 基金项目： | 国家医药科学技术博士点创新基金;国家自然科学基金 |
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| 摘 要： | 以西红花的球茎，叶片和花柱为外植体诱导愈伤组织，叶片诱导培养形成白色膨大组织，球茎培养得到松散型白色愈伤组织，诱导球茎愈伤的培养基为5mg/L 6-BA 8mg/L NAA，在球茎愈伤组织中的紫外部分没有检测到西红花甙，西红花醛和西红花苦甙，在花柱愈伤组织中检测到西红花醛，适合于继代和悬浮培养，经悬浮培养后，在培养液中可检测到西红花醛和西红花苦甙，悬浮培养有利于次和一代谢的积。
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| 关 键 词： | 西红花 花柱愈伤组织 继代培养 悬浮培养 |
| 文章编号： | 0490-6756(2001)03-0421-04 |
INDUCTION OF CALLUS AND SUBCULTURE AND SUSPENSION CULTURE OF STYLE CALLUS OF SAFFRON ( CROCUS SATIVUS L) |
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| ZHAO Jun,XU Ying,YAN Fang,TANG Lin,CHEN Fang.INDUCTION OF CALLUS AND SUBCULTURE AND SUSPENSION CULTURE OF STYLE CALLUS OF SAFFRON ( CROCUS SATIVUS L)[J].Journal of Sichuan University (Natural Science Edition),2001,38(3):421-424. |
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| Authors: | ZHAO Jun XU Ying YAN Fang TANG Lin CHEN Fang |
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| Abstract: | With corms, leaves and styles of Crocus sativus L as explants, callus was inducted. White expanding tissue was inducted from leaf culture. White loose callus was inducted from corm culture in medium 5mg/L 6 BA 8mg/L NAA. None of crocin, safranal and picrocrocin was tested from this white loose callus. Safranal was tested in style callus and it was suitable for subculture and suspension culture. Safranal and picrocrocin were tested after suspension culture. Suspension culture was favorable to the accumulation of secondary metabolism. |
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| Keywords: | Crocus sativus L style callus subculture suspension culture |
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