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| JAK-STAT/survivin通路对过氧化氢预处理诱导的适应性细胞保护作用的影响 | |
| 引用本文： | 陈秀琴,李景田,职君利,刘微,崔宇,冯鉴强.JAK-STAT/survivin通路对过氧化氢预处理诱导的适应性细胞保护作用的影响[J].韶关学院学报,2008,29(12). |
| 作者姓名： | 陈秀琴 李景田 职君利 刘微 崔宇 冯鉴强 |
| 作者单位： | 1. 韶关学院医学院,广东韶关,512026 2. 广东药学院临床医学院,广东广州,510180;中山大学基础医学院,广东广州,510080 3. 中山大学基础医学院,广东广州,510080 |
| 基金项目： | 韶关市科技计划项目(韶科(卫)2006-36号) |
| 摘 要： | 为了探讨H_2O_2预处理对存活素(survivin)表达的影响及JAK-STAT/survivin通路在H_2O_2预处理的适应性细胞保护中的作用,用PC12细胞建立H_2O_2预处理对抗H_2O_2诱导细胞凋亡的实验模型,应用甲氮甲哇蓝(MTT)法检测细胞存活率,碘化丙啶(PI)染色流式细胞术检测细胞凋亡率,Hoechst染色检测细胞凋亡的形态学改变,免疫印迹法(Western blot)测定survivin表达水平.结果显示:用100μmol/L H_2O_2预处理PC12细胞90min可明显地抑制300μmol/L H_2O_2作用12 h后引起的细胞毒性和细胞凋亡,并可以显著地促进PC12细胞survivin的表达;JAK_2抑制剂AG-490不仅可以抑制survivin的表达.而且能拮抗H_2O_2预处理的适应性细胞保护作用.研究表明,survivin是JAK-STAT通路的靶基因,JAK-STAT/survivin通路在H_2O_2预处理诱导的适应性细胞保护机制中起着重要的作用. |
| 关 键 词： | JAK-STAT通路 存活素 过氧化氢 预处理 细胞保护 |
The influence of JAK-STAT/survivin pathway in the adaptive cytoprotection induced by H2O2 preconditioning |
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| CHEN Xiu-qin,LI Jing-tian,ZHI Jun-li,LIU Wei,CUI Yu,FENG Jian-qiang.The influence of JAK-STAT/survivin pathway in the adaptive cytoprotection induced by H2O2 preconditioning[J].Journal of Shaoguan University(Social Science Edition),2008,29(12). | |
| Authors: | CHEN Xiu-qin LI Jing-tian ZHI Jun-li LIU Wei CUI Yu FENG Jian-qiang |
| Institution: | 1.Medical College; Shaoguan University; Shaoguan 512026; Guangdong; China; 2.Clinical Medical College; Guangdong College of Pharmacy; Guangzhou 510182; 3.Basis Medical College; Sun Yat-sen University; Guangzhou 510080; China); |
| Abstract: | The influence of H_2O_2 preconditioning on survivin expression and the role of JAK-STAT/survivin pathway in the adaptive cytoprotection induced by H_2O_2 preconditioning in PC12 cells were investigated.In PC12 cells setting up the experimental model of cytoprotection of H_2O_2 preconditioning against apoptosis induced by H_2O_2.The viability of PC12 cells was assessed by MTT assay,the percent of apoptotie cells was measured by propidium iodide stain flow cytometry(FCM),the morphological change of apoptotic ... |
| Keywords: | JAK-STAT pathway survivin hydrogen peroxide preconditiong cytoprotection |
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