| 纤维素酶曲的浅盘生产研究 |
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| 引用本文： | 张加春,易琴,黄遵锡.纤维素酶曲的浅盘生产研究[J].云南师范大学学报(自然科学版),2000,20(2):50-52. |
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| 作者姓名： | 张加春 易琴 黄遵锡 |
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| 作者单位： | 云南师范大学生命科学系,云南,昆明,650092 |
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| 摘 要： | 本试验以里氏木霉Ｔｒ－Ｈ为出发菌株,对其浅盘培养条件及酶活测定方法进行优化,结果,当接种量为０．３％,初始ＰＨ值２．０,料层厚度２．５ｃｍ时,２８℃培养１６０小时,５０℃浸提,酶活从原来的２８０ｍｇＧ／ｇ．ｈ提高到６００ｍＧ／ｇ．ｈ;扣盘使酶活下降。
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| 关 键 词： | 里氏木霉 纤维素酶 酶活 浅盘生产 |
| 修稿时间： | 1999-09-24 |
Studies on the Conditions for Cellulase Production from A Trichoderma reesei Strain |
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| ZHANG Jia-chun,YI Qin,HUANG Zun-xi.Studies on the Conditions for Cellulase Production from A Trichoderma reesei Strain[J].Journal of Yunnan Normal University (Natural Sciences Edition),2000,20(2):50-52. |
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| Authors: | ZHANG Jia-chun YI Qin HUANG Zun-xi |
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| Abstract: | A Trichoderma reesei strain Tr-H with
high potency for cellulase production was studied in this paper. The results showed that
the best conditions for producing cellulase were inoculating quantity 0.5%, original
pH2.0, material thickness 2.5 cm, culture temperature 28℃, extraction temperature 50℃, cultured at that condition for 160 hours,the filter
paper enzyme activity raised from 280mgG/g.h to 600mgG/g.h. |
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