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虎奶菇Pleurotus tuber-regium深层发酵的研究
引用本文:林跃鑫,刘春辉,肖良建,黄谚谚.虎奶菇Pleurotus tuber-regium深层发酵的研究[J].龙岩学院学报,2009,27(2).
作者姓名:林跃鑫  刘春辉  肖良建  黄谚谚
作者单位:1. 龙岩学院,福建龙岩,364000;福建师范大学,福建福州,350007
2. 厦门隆力德环境技术开发有限公司,福建厦门
3. 福建师范大学,福建福州,350007
摘    要:报道了虎奶菇Pleurotus tuber-regium深层发酵的最适培养基和摇瓶发酵条件.试验结果表明,Pleurotus tuber-regium对单糖、双糖、多糖和糖醇都有一定的利用,但葡萄糖、麦芽糖等对菌丝生长效果较好;酵母粉、黄豆饼粉等蛋白质含量高的氮源对菌丝的生长较为有利;通过k9(34)正交试验确定了Pleurotus tuber-regium发酵的最适培养基为:5%葡萄糖,0.4%酵母粉.0.1%KH2PO4,0.3%MgSO4·7H2O,0.001%VB1及微量NaCl;一定浓度内Na+对茵丝体生长有促进效果.最适的摇瓶发酵条件为:培养基起始pH7.0,培养温度32℃,接种量15%,摇床转速150r/min,250mL三角瓶最适装液量为70mL,发酵周期为6d.

关 键 词:深层发酵  培养条件  菌丝体

Study on submerged fermentation of Pleurotus tuber-regium
LIN Yue-xin,LIU Chun-hui,XIAO Lian-jian,HUANG Yan-yan.Study on submerged fermentation of Pleurotus tuber-regium[J].Journal of Longyan Univercity,2009,27(2).
Authors:LIN Yue-xin  LIU Chun-hui  XIAO Lian-jian  HUANG Yan-yan
Abstract:The optimal composition of culture media and cultivating conditions for submerged fermentation of Pleurotus tuber-regiun were explored.The results showed that glucose,maltose and yeast extracts,residue of soybean are the most favorite carbon and nitrogen resources,respectively,for mycelium growth of Pleurotus tuber-regiun in submerged culture.The suggest composition of culture medium is 5% glucose,0.4% yeast extract,0.1%KH2PO4,0.3% MgSO4.7H2O,0.001%VB1.NaCl can stimulate the mycelium growth.The optimal cond...
Keywords:Pleurotus tuber-regium
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