| 两株产纤维素酶细菌的分离和筛选 |
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| 引用本文： | 燕红,杨谦,王希国.两株产纤维素酶细菌的分离和筛选[J].黑龙江大学自然科学学报,2007,24(2):211-213. |
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| 作者姓名： | 燕红 杨谦 王希国 |
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| 作者单位： | 1. 哈尔滨工业大学,生命科学与工程系,黑龙江,哈尔滨,150001;哈尔滨理工大学,化学与环境工程学院,黑龙江,哈尔滨,150080
2. 哈尔滨工业大学,生命科学与工程系,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金资助项目(50178021) |
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| 摘 要： | 从林区土壤中分离能够分解纤维素的细菌菌株共15株,采用刚果红染色的初筛平板获取透明圈较明显的菌株8株。在此基础上,进行液体培养并测定酶活,得到2株酶活较高的菌株,分别编号为X18和X10-1-2。这两株菌均为G( )、形态杆状、可产芽孢、好氧,初步鉴定为芽孢杆菌属的两个不同种。
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| 关 键 词： | 纤维素酶 细菌 筛选 |
| 文章编号： | 1001-7011(2007)02-0211-03 |
| 修稿时间： | 2006年3月16日 |
Isolation and selection of two strains of bacteria producing cellulase |
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| YAN Hong,YANG Qian,WANG Xi-guo.Isolation and selection of two strains of bacteria producing cellulase[J].Journal of Natural Science of Heilongjiang University,2007,24(2):211-213. |
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| Authors: | YAN Hong YANG Qian WANG Xi-guo |
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| Institution: | 1.Department of Life Science and Engineering;Harbin Institute of Technology;Harbin 150001;China;2.College of Chemical and Environmental Engineering;Harbin University of Science and Technology;Harbin 150040;China |
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| Abstract: | The 15 strains of bacteria producing cellulase were isolated from soil.Eight colonies surrounded by some distinct zones of clearing in cellulose-congo red agar medium from the 15 strains above were obtained.By measuring the enzyme activity of liquid culture filtrates,two strains(termed X18 and X10-1-2,respectively) with higher cellulase activity were obtained.They are gram positive,endospore forming and aerobic bacteria.They all belong to the Bacillus genus. |
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| Keywords: | cellulase bacteria screening |
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