| Genetic relationships among Chinese and American isolates of Phytophthora sojae assessed by RAPD markers |
| |
| 作者姓名： | WANG Ziying WANG Yuanchao ZHANG Zhengguang ZHENG Xiaobuo |
| |
| 作者单位： | [1]Department of Plant Pathology, Nanjing Agricultural University,Nanjing 210095, China; [2]Department of biology, Anhui Institute of Education, Hefei 230061,China |
| |
| 基金项目： | Acknowledgements We thank Dr. Brett Tyler from Virginia Polytech and State University for providing isolates of Phytophthora soja. This work was supported by the China National Basic Research Program （Grant No. 2002CB 111400）. |
| |
| 摘 要： | The genetic diversity of three geographic populations of Phytophthora sojae from China and the United States was determined using random amplified polymorphic DNA （RAPD）. The purpose was to explore genetic relationships among Chinese and American isolates of the organism. 21 random primers were selected among 200 random primers screened. A total of 223 reproducible RAPD fragments were scored among 111 individuals, of which 199 （89.23%） were polymorphic. Analysis of genetic variation showed that there existed higher genetic variation in the United States population in comparison to the Chinese populations. Nei＇s genetic identity and principal component analysis indicated that the populations of Fujian and United States are closer to each other than to Heilongjiang populations. Shannon-Wiener diversity index revealed that the United States populations have a higher genetic di- versity than that of Chinese populations. These data are in support of the hypothesis that P. sojae in the United States might not have been introduced from China.
|
| 关 键 词： | 植物生理学 遗传多样性 地理分布 RAPD |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006-03-202006-07-03 |
Genetic relationships among Chinese and American isolates of Phytophthora sojae assessed by RAPD markers |
| |
| WANG Ziying WANG Yuanchao ZHANG Zhengguang ZHENG Xiaobuo.Genetic relationships among Chinese and American isolates of Phytophthora sojae assessed by RAPD markers[J].Chinese Science Bulletin,2006,51(17):2095-2102. |
| |
| Authors: | Ziying Wang Yuanchao Wang Zhenggung Zhang Xiaobuo Zheng |
| |
| Institution: | (1) Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China;(2) Department of biology, Anhui Institute of Education, Hefei, 230061, China |
| |
| Abstract: | The genetic diversity of three geographic populations of Phytophthora sojae from China and the United States was determined using random amplified polymorphic DNA (RAPD). The purpose was to explore
genetic relationships among Chinese and American isolates of the organism. 21 random primers were selected among 200 random
primers screened. A total of 223 reproducible RAPD fragments were scored among 111 individuals, of which 199 (89.23%) were
polymorphic. Analysis of genetic variation showed that there existed higher genetic variation in the United States population
in comparison to the Chinese populations. Nei’s genetic identity and principal component analysis indicated that the populations
of Fujian and United States are closer to each other than to Heilongjiang populations. Shannon-Wiener diversity index revealed
that the United States populations have a higher genetic diversity than that of Chinese populations. These data are in support
of the hypothesis that P. sojae in the United States might not have been introduced from China. |
| |
| Keywords: | Phytophthora sojae genetic diversity origin geographic distribution |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
