| 猪血管脱细胞细胞外基质制备的初步探讨 |
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| 引用本文： | 赵瑛.猪血管脱细胞细胞外基质制备的初步探讨[J].重庆师范大学学报(自然科学版),2006,23(1):61-63. |
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| 作者姓名： | 赵瑛 |
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| 作者单位： | 重庆师范大学,重庆市动物生物学重点实验室,生命科学学院,重庆,400047 |
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| 基金项目： | 重庆师范大学校科研和教改项目 |
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| 摘 要： | 采用高渗、低渗溶液,结合去污剂—酶联合处理猪腹主动脉,进行组织学检查,从而制备血管脱细胞细胞外基质。结果表明:经该法处理的猪血管细胞全部脱除,细胞外基质保持完好,未见胶原纤维、弹性纤维有断裂现象。作者认为,高渗、低渗溶液结合去污剂—酶联合处理,是制备血管脱细胞细胞外基质理想的方法。
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| 关 键 词： | 脱细胞基质 猪血管 制备 |
| 文章编号： | 1672-6693(2006)01-0061-03 |
| 修稿时间： | 2005年7月19日 |
A Preliminary Study on Preparation of Acellular Vascular Matrix |
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| ZHAO Ying.A Preliminary Study on Preparation of Acellular Vascular Matrix[J].Journal of Chongqing Normal University:Natural Science Edition,2006,23(1):61-63. |
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| Authors: | ZHAO Ying |
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| Abstract: | To carry out the preparation of acellular matrix from pigs,hypotonic and hypertonic solutions,detergent and proteinase were applied for a multistep process in this study.Morphological analyses indicated that the cells were removed effectively from pig aorta,the elastic fibers and collagen fibers were preserved.Acellular matrix prepared from multistage cell removing can be used as the scaffold to construct tissue engineering vessels. |
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| Keywords: | acellular matrix pig vessels preparation |
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