| Characterization of enhancer trap and gene trap harboring Ac／Ds transposon in transgenic rice |
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| 作者姓名： | Jin WZ Wang SM Xu M Duan RJ Wu P |
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| 作者单位： | TheStateKeyLaboratoryofPlantPhysiologyandBiochemistry,CollegeofLifeSciences,ZhejiangUniversity,Hangzhou310029,China |
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| 摘 要： | Insertion mutagenesis has become one of the most popular methods for gene functions analysis.Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice.The excision of Ds element was examined by PCR amplification.The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents.Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice.The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots,flowers or seeds.The GUS positive lines will be useful for identifying gene function in rice.
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| 关 键 词： | 转基因技术 稻 基因标记 基因捕集 |
Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice |
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| Jin WZ,Wang SM,Xu M,Duan RJ,Wu P.Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice[J].Journal of Zhejiang University Science,2004,5(4):390-399. |
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| Authors: | Jin Wei-zheng Wang Shao-min Xu Min Duan Rui-jun Wu Ping |
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| Institution: | The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China. |
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| Abstract: | Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F(2) populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. |
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